ALPL - Alkaline phosphatase tissue-nonspecific isozyme |Elisa - Clia - Antibody - Protein

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Background:

Alkaline phosphatase, tissue-nonspecific isozyme (ALPL) is an enzyme encoded by the ALPL gene in humans. It is also commonly known as tissue-nonspecific alkaline phosphatase (TNAP). ALPL is a type of alkaline phosphatase found in bone, liver, kidney, and intestine. ALPL plays a crucial role in bone mineralization and is involved in the regulation of phosphate metabolism. It catalyzes the hydrolysis of phosphate esters in an alkaline environment, leading to the release of inorganic phosphate. In bone tissue, ALPL is primarily expressed by osteoblasts, where it helps in the mineralization process by generating inorganic phosphate ions, which are essential for the formation of hydroxyapatite crystals, the main component of bone mineral. Deficiencies or mutations in the ALPL gene can lead to a rare genetic disorder known as hypophosphatasia (HPP). HPP is characterized by defective bone mineralization, resulting in weak and soft bones, skeletal abnormalities, and a range of other symptoms depending on the severity of the condition. These symptoms can include dental problems, respiratory issues, muscle weakness, and fractures. ALPL levels in the blood can also be used as a biomarker for certain diseases or conditions. For example, elevated levels of ALPL in the blood may indicate liver or bone disorders, while decreased levels may occur in conditions such as hypophosphatasia or severe malnutrition.


Protein Structure:

  • Domain Structure: ALPL consists of a single polypeptide chain that forms a homodimer. Each monomer has an active site containing a zinc ion necessary for catalytic activity. The structure includes a crown domain that plays a role in substrate binding and catalysis.
  • Glycosylation: The protein is glycosylated, which is essential for its stability and function.
  • Membrane Association: TNAP is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor, facilitating its access to substrates on the cell surface and in the extracellular matrix.


Classification and Subtypes:

  • Isozymes: TNAP is one of the four main types of alkaline phosphatases, the others being intestinal, placental, and germ cell ALPs. TNAP is distinct in its widespread expression and role in various tissues.
  • Genetic Variants: Different genetic variants of ALPL can lead to variations in enzyme activity and are associated with specific clinical conditions.


Function and Biological Significance:

  • Bone Mineralization: TNAP plays a crucial role in bone mineralization by hydrolyzing pyrophosphate, an inhibitor of mineralization, thus allowing calcium phosphate deposition in the bone matrix.
  • Detoxification: In the liver, TNAP is involved in the detoxification process by dephosphorylating various molecules.
  • Metabolism: It plays a role in phosphate metabolism, contributing to the regulation of phosphate levels in the body.


Interactions:

  • Substrates: TNAP interacts with a variety of substrates, including inorganic pyrophosphate, ATP, and phosphoproteins.

  • Protein Partners: TNAP interacts with other proteins involved in bone formation and mineralization, such as collagen and matrix Gla protein (MGP).


Clinical Issues:

  • Hypophosphatasia (HPP): Mutations in the ALPL gene cause hypophosphatasia, a rare inherited disorder characterized by defective bone mineralization, leading to rickets in children and osteomalacia in adults. Severity varies widely, from prenatal lethality to adult-onset forms.
  • Periodontal Disease: TNAP deficiency can also lead to dental issues, including premature loss of teeth and defective cementum formation.
  • Rheumatoid Arthritis: Elevated levels of TNAP have been associated with inflammatory conditions like rheumatoid arthritis, indicating its role in inflammation and immune responses.


Summary:

The tissue-nonspecific alkaline phosphatase (TNAP) is a crucial enzyme encoded by the ALPL gene, involved in dephosphorylation processes essential for bone mineralization, detoxification, and phosphate metabolism. Its wide expression in tissues such as bone, liver, and kidney underscores its significance in various physiological processes. Mutations in ALPL lead to hypophosphatasia, a disorder affecting bone and dental health. Understanding TNAP's structure, function, and interactions is vital for developing therapeutic strategies for conditions like hypophosphatasia and other related disorders.



ALPL Recommended name:

Alkaline phosphatase tissue-nonspecific isozyme (ALPL)

Aliases for ALPL

AP-TNAP,TNS-ALP

En la tabla siguiente se muestra una comparativa de todos los reactivos disponibles en nuestro catálogo (Proteins and Peptides, ELISA Kits, Primary Antibodies, CLIA Kits) relacionados con ALPL - Alkaline phosphatase tissue-nonspecific isozyme

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immunoassays

providerCodereferencenamereactivitysample typeassay typetest rangesensitivitypricesize 1uniprot idstatus
AbbexaALPLabx355955Chicken Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitChickenSerum, plasma and other biological fluids.Sandwich0.312 ng/ml - 20 ng/ml0.19 ng/ml643.596 testsRUO
AbbexaALPLabx515152Cow Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitCowTissue homogenates, cell lysates and other biological fluids.0.156 ng/ml - 10 ng/ml71596 testsP09487RUO
AbbexaALPLabx150629Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitHumanSerum, plasma, cell culture supernatants, tissue homogenates, cell lysates and other biological fluids.Sandwich0.78 ng/ml - 50 ng/ml< 0.3 ng/ml68996 testsRUO
AbbexaALPLabx196486Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) CLIA KitHumanSerum, plasma and other biological fluids.Sandwich15.6 pg/ml - 1000 pg/ml9.38 pg/ml643.596 testsRUO
AbbexaALPLabx492404Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) CLIA KitHumanSerum, plasma and other biological fluids.Sandwich78 pg/ml - 5000 pg/ml< 31 pg/ml84596 testsRUO
AbbexaALPLabx575206Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitHumanTissue homogenates, cell lysates and other biological fluids.Competitive3.12 mU/ml - 200 mU/ml< 1.34 mU/ml55996 testsP05186RUO
AbbexaALPLabx352245Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitHumanTissue homogenates, cell lysates and other biological fluids.Sandwich78 pg/ml - 5000 pg/ml46.9 pg/ml58596 testsP05186RUO
AbbexaALPLabx353364Monkey Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitMonkeySerum, plasma and other biological fluids.Sandwich0.156 ng/ml - 10 ng/ml0.1 ng/ml58596 testsRUO
AbbexaALPLabx196755Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) CLIA KitMouseSerum, plasma and other biological fluids.Sandwich31.2 pg/ml - 2000 pg/ml18.8 pg/ml643.596 testsRUO
AbbexaALPLabx255208Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitMouseTissue homogenates, cell lysates and other biological fluids.Sandwich0.156 ng/ml - 10 ng/ml0.1 ng/ml578.596 testsP09242RUO
AbbexaALPLabx492405Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) CLIA KitMouseSerum, plasma, tissue homogenates and other biological fluids.Sandwich0.156 ng/ml - 10 ng/ml< 0.062 ng/ml84596 testsRUO
AbbexaALPLabx153626Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitMouseSerum, plasma, tissue homogenates and other biological fluids.Sandwich0.156 ng/ml - 10 ng/ml< 0.07 ng/ml643.596 testsRUO
AbbexaALPLabx573896Rabbit Alkaline Phosphatase, Tissue-nonspecific (ALPL) ELISA KitRabbitTissue homogenates, cell lysates and other biological fluids.Sandwich0.312 ng/ml - 20 ng/ml< 0.115 ng/ml71596 testsRUO
AbbexaALPLabx155186Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitRatSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.Sandwich3.12 ng/ml - 200 ng/ml< 1.15 ng/ml71596 testsRUO
AbbexaALPLabx196756Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) CLIA KitRatSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.Sandwich0.27 ng/ml - 200 ng/ml< 0.12 ng/ml84596 testsRUO
AbbexaALPLabx256730Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA KitRatSerum, plasma and other biological fluids.Sandwich0.625 ng/ml - 40 ng/ml0.38 ng/ml58596 testsRUO

Primary Antibodies

providerCodereferencenamereactivityclonalityhostimmunogen targetisotypeconjugationtested applicationspricesize 1uniprot idstatus
FineTestALPLFNab10377anti- ALPL antibodyhumanpolyclonalRabbitalkaline phosphatase, liver/bone/kidneyIgGUnconjugatedELISA, WB, IHC100µgP05186RUO
FineTestALPLFNab00347anti- ALPL antibodyhumanpolyclonalRabbitalkaline phosphatase, liver/bone/kidneyIgGUnconjugatedELISA, WB, IHC, FC100µgP05186RUO
AbbexaALPLabx109514Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, IHC162.520 µgP05186RUO
AbbexaALPLabx011924Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanMonoclonalMouseAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, WB, IHC, FCM362.5100 µlP05186RUO
AbbexaALPLabx230347Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, WB, IHC, FCM350100 µgP05186RUO
AbbexaALPLabx146588Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, WB, IHC350100 µgP05186RUO
AbbexaALPLabx431085Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalGoatAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedP-ELISA, WB387.5200 µlRUO
AbbexaALPLabx128756Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedWB, IHC, IF/ICC262.5100 µlP05186RUO
AbbexaALPLabx171157Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanMonoclonalMouseAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedWB, IHC, IF/ICC275100 µlRUO
AbbexaALPLabx320512Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, IHC237.550 µlP05186RUO
AbbexaALPLabx105117Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (Biotin)HumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)BiotinELISA162.520 µgP05186RUO
AbbexaALPLabx106534Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (FITC)HumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)FITC162.520 µgP05186RUO
AbbexaALPLabx107951Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (HRP)HumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)HRPELISA162.520 µgP05186RUO
AbbexaALPLabx370908Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody PairHumanAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)ELISA10755 × 96 testsRUO
AbbexaALPLabx028274Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, WB, IHC, IF/ICC, FCM281.2580 µlP05186RUO
AbbexaALPLabx327885Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, WB187.550 µgP05186RUO
AbbexaALPLabx001003Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedWB, IHC17520 µlP05186RUO
AbbexaALPLabx015103Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, WB43.7510 µgP05186RUO
AbbexaALPLabx242417Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, IHC25050 µlP05186RUO
AbbexaALPLabx242425Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, IHC25050 µlP05186RUO
AbbexaALPLabx110934Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, WB, IHC612.5100 µlP05186RUO
AbbexaALPLabx140892Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanMonoclonalMouseAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedIF/ICC, FCM3500.1 mgP05186RUO
AbbexaALPLabx140910Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (PE)HumanMonoclonalMouseAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)PEFCM4500.1 mgP05186RUO
AbbexaALPLabx140901Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (APC)HumanMonoclonalMouseAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)APCFCM4500.1 mgP05186RUO
AbbexaALPLabx330131Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyHumanPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA, WB187.550 µgP05186RUO
AbbexaALPLabx272770Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (Biotin)MousePolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)BiotinWB, IHC, IF/ICC362.5200 µlRUO
AbbexaALPLabx104048Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyMousePolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedWB, IHC, IF/ICC262.5100 µlP09242RUO
AbbexaALPLabx107952Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (HRP)MousePolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)HRPELISA162.520 µgP09242RUO
AbbexaALPLabx105118Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (Biotin)MousePolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)BiotinELISA162.520 µgP09242RUO
AbbexaALPLabx106535Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (FITC)MousePolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)FITC162.520 µgP09242RUO
AbbexaALPLabx109515Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyMousePolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedELISA162.520 µgP09242RUO
AbbexaALPLabx273949Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Antibody (FITC)MousePolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)FITCWB, IHC, IF/ICC425200 µlRUO
AbbexaALPLabx117584Alkaline Phosphatase, Tissue-Nonspecific Isozyme (Alpl) Antibody PairMouseAlkaline Phosphatase, Tissue-Nonspecific Isozyme (Alpl)ELISA9505 × 96 testsRUO
AbbexaALPLabx171158Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyRatMonoclonalMouseAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedWB, IHC, IF/ICC812.51 mlRUO
AbbexaALPLabx175321Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) AntibodyRatPolyclonalRabbitAlkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)UnconjugatedWB, IHC, IF/ICC275100 µlRUO

Proteins and Peptides

providerCodereferencenameoriginexpressionhostconjugationtested applicationspricesize 1uniprot idstatus
AbbexaALPLabx065219Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ProteinMouseRecombinantE. coliUnconjugatedWB, SDS-PAGE22510 µgP09242RUO
FineTestALPLP0476Recombinant Human ALPLHumanRecombinantE.ColiWestern Blot, ELISA50μgP05186RUO
AbbexaALPLabx652457Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ProteinRatRecombinantE. coliUnconjugatedWB, SDS-PAGE20010 µgRUO
AbbexaALPLabx694255Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ProteinMouseRecombinantHEK293 cellsSDS-PAGE30020 µgP09242RUO
AbbexaALPLabx166663Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ProteinHumanRecombinantE. coliUnconjugatedWB, SDS-PAGE212.510 µgRUO

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