Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Protein
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935106861
info@markelab.com
Precio
234€ (10 µg)
Protein ALPL
proveedor
Abbexareference
abx065219Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Recombinant Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).
Background
Alkaline phosphatase, tissue-nonspecific isozyme (ALPL) is an enzyme encoded by the ALPL gene in humans. It is also commonly known as tissue-nonspecific alkaline phosphatase (TNAP). ALPL is a type of alkaline phosphatase found in bone, liver, kidney, and intestine. ALPL plays a crucial role in bone mineralization and is involved in the regulation of phosphate metabolism. It catalyzes the hydrolysis of phosphate esters in an alkaline environment, leading to the release of inorganic phosphate. In bone tissue, ALPL is primarily expressed by osteoblasts, where it helps in the mineralization process by generating inorganic phosphate ions, which are essential for the formation of hydroxyapatite crystals, the main component of bone mineral. Deficiencies or mutations in the ALPL gene can lead to a rare genetic disorder known as hypophosphatasia (HPP). HPP is characterized by defective bone mineralization, resulting in weak and soft bones, skeletal abnormalities, and a range of other symptoms depending on the severity of the condition.
Características del producto
category
Proteins and Peptides
origin
Mouse
host
E. coli
immunogen target
Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 37.2 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Phe18-Arg335 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
ALPL
dry ice
No
availability
Shipped within 5-12 working days.
alias
AP-TNAP,TNS-ALP
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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