Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Protein

Este producto es parte de ALPL - Alkaline phosphatase tissue-nonspecific isozyme
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208€ (10 µg)

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935106861
info@markelab.com
name
Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx652457
tested applications
WB, SDS-PAGE

Description

Rat Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) Protein is a Recombinant Rat protein expressed in E. coli.

Documents del producto

Instrucciones
Data sheet
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Product specifications

Category
Proteins and Peptides
Immunogen Target
Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL)
Host
E. coli
Origin
Rat
Conjugation
Unconjugated
Observed MW
Molecular Weight: Calculated MW: 36.9 kDa  Observed MW: 37 kDa
Concentration: Prior to lyophilization: 200 µg/ml
Sequence Fragment: Phe18-Arg335
Tag: N-terminal His tag
Expression
Recombinant
Purity
> 90%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Dry Ice
No
Alias
AP-TNAP,TNS-ALP
Background
Protein ALPL
Status
RUO
Note
This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

Alkaline phosphatase, tissue-nonspecific isozyme (ALPL) is an enzyme encoded by the ALPL gene in humans. It is also commonly known as tissue-nonspecific alkaline phosphatase (TNAP). ALPL is a type of alkaline phosphatase found in bone, liver, kidney, and intestine. ALPL plays a crucial role in bone mineralization and is involved in the regulation of phosphate metabolism. It catalyzes the hydrolysis of phosphate esters in an alkaline environment, leading to the release of inorganic phosphate. In bone tissue, ALPL is primarily expressed by osteoblasts, where it helps in the mineralization process by generating inorganic phosphate ions, which are essential for the formation of hydroxyapatite crystals, the main component of bone mineral. Deficiencies or mutations in the ALPL gene can lead to a rare genetic disorder known as hypophosphatasia (HPP). HPP is characterized by defective bone mineralization, resulting in weak and soft bones, skeletal abnormalities, and a range of other symptoms depending on the severity of the condition.

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