human Leptin Elisa
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Description
The ELISA E07 is intended to be used for quantitative measurement of human Leptin in human serum and plasma samples. This enzyme immunoassay kit is suited for measuring human leptin in serum or plasma, and conditioned adipocyte culture media for scientific and diagnostic purposes. Measuring leptin in anorectic or cachectic patients, young children or in specimen other than serum, such as urine, cerebrospinal fluid, and certain cell culture media, is also possible with this kit.
Documents del producto
Product specifications
| Category | ELISA Kits |
| Reactivity | Human |
| Assay Data | Reference Material: NIBSC 97/594 Incubation Period: 1.75 h Calibrator: recom. Leptin Controls: 2 Controls, freeze-dried |
| Assay Type | Sandwich |
| Test Range | 0.25 – 100 µg/L |
| Sensitivity | < 0.25 µg/L |
| Recommended Dilution | undiluted |
| Origin | Antibody: Mouse |
| Size 1 | 96 det. |
| Tested Applications | Elisa |
| Sample Type | Serum/ Plasma |
| Availability | 3-5 days. |
| Storage | Shelf life: between 1 and 2 years Store the kit at 2-8°C after receipt until its expiry date |
| Alias | Leptin (Obesity factor) |
| Background | Elisa Kits for: LEP |
| Status | IVD |
| Note | Intra/Interassay Variance [%]<15 |
Descripción
El human Leptin Elisa kit REf: E07 está diseñado para la medición cuantitativa de leptina humana en muestras de suero y plasma humanos. Este kit de inmunoensayo enzimático es adecuado para la medición de leptina humana en suero, plasma y medios de cultivo de adipocitos acondicionados con fines científicos y diagnósticos. Este kit también permite la medición de leptina en pacientes anoréxicos o caquécticos, niños pequeños o en muestras distintas del suero, como orina, líquido cefalorraquídeo y ciertos medios de cultivo celular.
Leptin is a single-chain proteohormone with a molecular weight of 16 kD, which is thought to play a key role in the regulation of body weight. Its amino acid sequence exhibits no major homologies with other proteins. Leptin is almost exclusively produced by differentiated adipocytes. It acts on the central nervous system, in particular the hypothalamus, thereby suppressing food intake and stimulating energy expenditure . Leptin receptors - alternatively spliced forms exist that differ in length - belong to the cytokine class I receptor family. They are found ubiquitously in the body, indicating a general role of leptin. A circulating form of the leptin receptor exists which acts as one of several leptin binding proteins. Besides its metabolic effects, leptin was shown to have a strong influence on a number of endocrine axes.
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