Human Resistin ELISA

Product specifications

ELISA Kits

Human

Reference Material: recombinant resistin
Incubation Period: 4h
Calibrator: recombinant resistin
Controlrs: 2 Controls, lyophilised

Sandwich

0.0121 – 21 µg/L

<0.0121 µg/L

1:21

Antibody: Rabbit

96 det.

Elisa

Serum
EDTA / Heparin Plasma

3-5 days.

Shelf life: between 1 and 2 years
Store the kit at 2-8°C after receipt until its expiry date

adipose tissue-specific secretory factor ,ADSF

Elisa Kits for: RETN

IVD

Intra/Interassay Variance [%]<10

Descripción

Human Resisitin ELISA kit permite al usuario determinar la concentración exacta de resistina en suero/plasma humano, así como en otros fluidos corporales y, de este modo, ayuda a la investigación de la biología de la resistina.

Resistin, a cysteine-rich protein of 11.3 kDa, was firstly found in mice and constitutes together with RELMα RELMß and RELMγ the protein family of resistin-like molecules (RELM). In humans, resistin and RELMß but no other proteins of the RELM family were found. The human form of resistin shows a homology of 53% to the murine protein. It has 11 cysteineresidues, is synthesized as a propetide of 108 amino acids and secreted as a dimer, build by a disulfide bridge of cysteine residues. Beside this intermolecular disulfide bridge, 5 additional intramolecular ones exist. Appearance of multi- and oligomer formation was proved by size exclusion chromatography. Thereby it was shown, that oligomer formation is SDS-insensitive but can be inhibited by βmercaptoethanol and is therefore likely to be caused by disulfide bridges. Further on, the resistin structure seems to be dependent on its concentration, as circular dichroism analysis shows a concentration dependent shift of α-helical to ß-sheet structure.

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