Functional Leptin Elisa
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Description
bioLEP ELISA a test for the measurement of biologically active leptinHere, the measurement is not based on two specific antibodies, but on the combination of a specific, polyclonal anit leptin antibody with the Leptin binding protein, the extracellular part of the leptin receptor. In this way, the test allows to measure only the functional leptin that is able to bind to the receptor
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Product specifications
| Category | ELISA Kits |
| Reactivity | Human |
| Assay Data | Reference Material: NIBSC 97/594 Incubation Period: 4 h Calibrator: recom. Leptin |
| Assay Type | Functional (Ligand-Enzyme Immunoassay) |
| Test Range | 1 – 120 µg/L |
| Sensitivity | 0.01 µg/L |
| Recommended Dilution | 1:20 |
| Origin | Antibody: Rabbit |
| Size 1 | 96 det. |
| Tested Applications | Elisa |
| Sample Type | Serum/ Plasma |
| Availability | 3-5 days. |
| Storage | Shelf life: between 1 and 2 years Store the kit at 2-8°C after receipt until its expiry date |
| Alias | Leptin (Obesity factor) |
| Background | Elisa Kits for: LEP |
| Status | RUO |
| Note | Intra/Interassay Variance [%]<10 |
Descripción
bioLEP ELISA es una prueba para la medición de leptina biológicamente activa. La medición no se basa en dos anticuerpos específicos, sino en la combinación de un anticuerpo policlonal específico anti-leptina con la proteína de unión a leptina, la parte extracelular del receptor de leptina. De esta manera, la prueba permite medir únicamente la leptina funcional capaz de unirse al receptor.
Leptin a hormone of 146 amino acids shows a molecular weight of 16 kDa (P41159). It consists of four anti-parallel α-helices. Leptin is primarily produced by adipocytes and thus provides a signal to the energy state of the organism. By binding to the leptin receptor (P48357) it influences the activity of the JAK -STAT pathway and thereby regulates energy metabolism, especially the food intake. Leptin levels show a circadian variation (+/- 30%) and are dependent on BMI, pubertal status, and gender. In addition to the expression itself, the bioavailability of leptin, is regulated via a binding protein, as with many hormones with type I cytokine receptors. The binding protein is the extracellular domain of the Leptin receptor formed by proteolytic cleavage of the receptor be metalloproteases (ADAM 10/17).
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