Human DNA Fragmentation Factor Subunit Alpha (DFFa) Protein

Este producto es parte de DFFA - DNA fragmentation factor subunit alpha
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234€ (10 µg)

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935106861
info@markelab.com
name
Human DNA Fragmentation Factor Subunit Alpha (DFFa) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx167049
tested applications
WB, SDS-PAGE

Description

DNA Fragmentation Factor Subunit Alpha Protein is a recombinant Human protein expressed in E. coli.

Documents del producto

Instrucciones
Data sheet
Descargar

Product specifications

Category
Proteins and Peptides
Immunogen Target
DNA Fragmentation Factor Subunit Alpha (DFFa)
Host
E. coli
Origin
Human
Conjugation
Unconjugated
Observed MW
Molecular Weight: Calculated MW: 32.1 kDa
Concentration: Prior to lyophilization: 200 µg/ml
Sequence Fragment: Ala51-Ser305
Tag: N-terminal His tag
Expression
Recombinant
Purity
> 95%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
O00273
Alias
DFF-45, DFF1, ICAD, Inhibitor of caspase-activated DNase,DFF45,DFFA
Background
Protein DFFA
Status
RUO
Note
This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

DNA fragmentation factor subunit alpha (DFFA) acts as the regulatory component of the DFF complex, forming a heterodimer with DFFB. DFFA functions primarily as an inhibitor of DFFB, maintaining it in an inactive state in healthy cells. Upon receiving apoptotic signals, caspases cleave DFFA, thereby releasing and activating DFFB. This cleavage event is a critical checkpoint in the apoptotic pathway, ensuring that DNA fragmentation occurs only during apoptosis. Beyond its role as a DFFB inhibitor, DFFA is implicated in nuclear condensation, which occurs concurrently with DNA fragmentation. Alterations in DFFA expression or function can influence the cell's ability to undergo programmed cell death, impacting both normal tissue homeostasis and disease processes, such as tumorigenesis or immune-related disorders.

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