Human DNA Fragmentation Factor Subunit Alpha (DFFa) Protein

Este producto es parte de DFFA - DNA fragmentation factor subunit alpha
Human DNA Fragmentation Factor Subunit Alpha (DFFa) Protein

Pida mas información

935106861

info@markelab.com

Precio

234€ (10 µg)

Protein DFFA

proveedor

Abbexa

reference

abx167049

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

DNA Fragmentation Factor Subunit Alpha Protein is a recombinant Human protein expressed in E. coli.


Background

DNA fragmentation factor subunit alpha (DFFA) acts as the regulatory component of the DFF complex, forming a heterodimer with DFFB. DFFA functions primarily as an inhibitor of DFFB, maintaining it in an inactive state in healthy cells. Upon receiving apoptotic signals, caspases cleave DFFA, thereby releasing and activating DFFB. This cleavage event is a critical checkpoint in the apoptotic pathway, ensuring that DNA fragmentation occurs only during apoptosis. Beyond its role as a DFFB inhibitor, DFFA is implicated in nuclear condensation, which occurs concurrently with DNA fragmentation. Alterations in DFFA expression or function can influence the cell's ability to undergo programmed cell death, impacting both normal tissue homeostasis and disease processes, such as tumorigenesis or immune-related disorders.

Características del producto

category

Proteins and Peptides


origin

Human


host

E. coli


immunogen target

DNA Fragmentation Factor Subunit Alpha (DFFa)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 32.1 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Ala51-Ser305 Tag: N-terminal His tag


expression

Recombinant


purity

> 95%


buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

DFFA


dry ice

No


availability

Shipped within 5-7 working days.


alias

DFF-45, DFF1, ICAD, Inhibitor of caspase-activated DNase,DFF45,DFFA


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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reactivity

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clonality

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DNA Fragmentation Factor Subunit Alpha (DFFA) Antibody

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proveedor

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reference

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Tested Applications

ELISA, WB, IF/ICC

reactivity

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status

RUO

clonality

Polyclonal

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