Human DNA Fragmentation Factor Subunit Alpha (DFFa) Protein
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935106861
info@markelab.com
Precio
234€ (10 µg)
Protein DFFA
proveedor
Abbexareference
abx167049Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
DNA Fragmentation Factor Subunit Alpha Protein is a recombinant Human protein expressed in E. coli.
Background
DNA fragmentation factor subunit alpha (DFFA) acts as the regulatory component of the DFF complex, forming a heterodimer with DFFB. DFFA functions primarily as an inhibitor of DFFB, maintaining it in an inactive state in healthy cells. Upon receiving apoptotic signals, caspases cleave DFFA, thereby releasing and activating DFFB. This cleavage event is a critical checkpoint in the apoptotic pathway, ensuring that DNA fragmentation occurs only during apoptosis. Beyond its role as a DFFB inhibitor, DFFA is implicated in nuclear condensation, which occurs concurrently with DNA fragmentation. Alterations in DFFA expression or function can influence the cell's ability to undergo programmed cell death, impacting both normal tissue homeostasis and disease processes, such as tumorigenesis or immune-related disorders.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
DNA Fragmentation Factor Subunit Alpha (DFFa)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 32.1 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Ala51-Ser305 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
DFFA
dry ice
No
availability
Shipped within 5-7 working days.
alias
DFF-45, DFF1, ICAD, Inhibitor of caspase-activated DNase,DFF45,DFFA
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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