DNA Fragmentation Factor Subunit Alpha (DFFA) Antibody
292.5€ (80 µl)
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name
DNA Fragmentation Factor Subunit Alpha (DFFA) Antibody
category
Primary Antibodies
provider
Abbexa
reference
abx034309
tested applications
ELISA, WB
Description
DFFA is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | DNA Fragmentation Factor Subunit Alpha (DFFA) |
| Host | Rabbit |
| Reactivity | Human |
| Recommended Dilution | WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified through a protein A column, followed by peptide affinity purification. |
| Size 1 | 80 µl |
| Size 2 | 400 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | O00273 |
| Alias | DFF-45, DFF1, ICAD, Inhibitor of caspase-activated DNase,DFF45,DFFA |
| Background | Antibody anti-DFFA |
| Status | RUO |
Descripción
DNA fragmentation factor subunit alpha (DFFA) acts as the regulatory component of the DFF complex, forming a heterodimer with DFFB. DFFA functions primarily as an inhibitor of DFFB, maintaining it in an inactive state in healthy cells. Upon receiving apoptotic signals, caspases cleave DFFA, thereby releasing and activating DFFB. This cleavage event is a critical checkpoint in the apoptotic pathway, ensuring that DNA fragmentation occurs only during apoptosis. Beyond its role as a DFFB inhibitor, DFFA is implicated in nuclear condensation, which occurs concurrently with DNA fragmentation. Alterations in DFFA expression or function can influence the cell's ability to undergo programmed cell death, impacting both normal tissue homeostasis and disease processes, such as tumorigenesis or immune-related disorders.
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