Human 2-(3-Amino-3-Carboxypropyl) Histidine Synthase Subunit 1 (DPH1) ELISA Kit

Product specifications

ELISA Kits

2-(3-Amino-3-Carboxypropyl) Histidine Synthase Subunit 1 (DPH1)

Human

Colorimetric

Quantitative

Sandwich

0.156 ng/ml - 10 ng/ml

< 0.094 ng/ml

Optimal dilutions/concentrations should be determined by the end user.

96 tests

Lyophilized

ELISA

Tissue homogenates, cell lysates and other biological fluids.

Shipped within 5-12 working days. The validity for this kit is 6 months.

Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

No

Q9BZG8

1801

603527

DPH2L,OVCA1,DEDSSH,DPH2L1,Diphthamide biosynthesis protein 1,Diphtheria toxin resistance protein 1,Ovarian cancer-associated gene 1 protein

Elisa kits for DPH1

RUO

Validity: The validity for this kit is 6 months.
This product is for research use only.   The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.   Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

Descripción

DPH1 is a key enzyme involved in the biosynthesis of diphthamide, a unique post-translational modification of histidine residue found exclusively on eukaryotic elongation factor 2 (eEF2). This modification is critical for cellular protein synthesis and is targeted by bacterial toxins such as diphtheria toxin and Pseudomonas exotoxin A. DPH1 functions as part of a complex multiprotein pathway that catalyzes the initial step of diphthamide synthesis, converting a specific histidine residue on eEF2 into an intermediate form. Genetic mutations or deletions in DPH1 have been implicated in various human diseases, including developmental disorders and cancer. DPH1's role extends beyond diphthamide biosynthesis, with studies suggesting its involvement in embryonic development, particularly neural tube closure, and hematopoiesis. Structurally, DPH1 contains conserved domains required for its catalytic activity and interactions with other diphthamide biosynthesis proteins like DPH2 and DPH3. Loss of DPH1 function impairs cellular sensitivity to diphtheria toxin, providing a functional assay for its activity. DPH1 is ubiquitously expressed, with the highest levels found in actively proliferating tissues. It also exhibits a conserved role in diphthamide synthesis across eukaryotes, underscoring its evolutionary significance.

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2-(3-Amino-3-Carboxypropyl)histidine Synthase Subunit 1 (DPH1) Antibody

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM].

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