Human Caspase Activated DNase (DFFB) Protein

Este producto es parte de DFFB - DNA fragmentation factor subunit beta
Human Caspase Activated DNase (DFFB) Protein

Pida mas información

935106861

info@markelab.com

Precio

234€ (10 µg)

Protein DFFB

proveedor

Abbexa

reference

abx065783

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Human Caspase Activated DNase (DFFB) Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).


Background

DNA fragmentation factor subunit beta (DFFB) is an essential component of the apoptotic machinery, functioning as a part of the DNA fragmentation factor (DFF) complex. DFFB forms a heterodimer with its partner subunit, DNA fragmentation factor subunit alpha (DFFA). This beta subunit possesses the enzymatic activity required for the cleavage of DNA into nucleosomal fragments, a hallmark of apoptosis. DFFB, a caspase-activated DNase, is kept in an inactive state by its binding to DFFA until caspases, triggered by apoptotic signals, cleave DFFA, releasing DFFB to execute its DNase function. Dysregulation of DFFB activity is associated with pathological conditions, including resistance to apoptosis in cancer and excessive cell death in degenerative diseases. DFFB activity is tightly regulated to ensure its apoptotic function is confined to specific cellular contexts.

Características del producto

category

Proteins and Peptides


origin

Human


host

E. coli


immunogen target

Caspase Activated DNase


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 31.1 kDa Observed MW (SDS-PAGE): 29 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Arg87-Lys323 Tag: N-terminal His tag


expression

Recombinant


purity

> 95%


buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

DFFB


dry ice

No


availability

Shipped within 5-7 working days.


alias

DFFB, DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase), CAD, CPAN, DFF-40, DFF2, DFF40, DNA fragmentation factor subunit beta


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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