Human Caspase Activated DNase (DFFB) Protein
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935106861
info@markelab.com
Precio
234€ (10 µg)
Protein DFFB
proveedor
Abbexareference
abx065783Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Human Caspase Activated DNase (DFFB) Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Background
DNA fragmentation factor subunit beta (DFFB) is an essential component of the apoptotic machinery, functioning as a part of the DNA fragmentation factor (DFF) complex. DFFB forms a heterodimer with its partner subunit, DNA fragmentation factor subunit alpha (DFFA). This beta subunit possesses the enzymatic activity required for the cleavage of DNA into nucleosomal fragments, a hallmark of apoptosis. DFFB, a caspase-activated DNase, is kept in an inactive state by its binding to DFFA until caspases, triggered by apoptotic signals, cleave DFFA, releasing DFFB to execute its DNase function. Dysregulation of DFFB activity is associated with pathological conditions, including resistance to apoptosis in cancer and excessive cell death in degenerative diseases. DFFB activity is tightly regulated to ensure its apoptotic function is confined to specific cellular contexts.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Caspase Activated DNase
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 31.1 kDa Observed MW (SDS-PAGE): 29 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Arg87-Lys323 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
DFFB
dry ice
No
availability
Shipped within 5-7 working days.
alias
DFFB, DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase), CAD, CPAN, DFF-40, DFF2, DFF40, DNA fragmentation factor subunit beta
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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