DNA Fragmentation Factor Subunit Beta (DFFB) Antibody
292.5€ (80 µl)
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Name
DNA Fragmentation Factor Subunit Beta (DFFB) Antibody
Category
Primary Antibodies
Provider
Abbexa
Reference
abx034537
Tested Applications
ELISA, WB, IHC, FCM
Description
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Primary Antibodies |
| Immunogen Target | Target: DNA Fragmentation Factor Subunit Beta (DFFB) Immunogen: KLH-conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DFFB. |
| Host | Rabbit |
| Reactivity | Human |
| Recommended Dilution | WB: 1/1000, IHC-P: 1/10 - 1/50, FCM: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Observed MW | Calculated MW: 39.1 kDa |
| Purification | Purified through a protein A column, followed by peptide affinity purification. |
| Size 1 | 80 µl |
| Size 2 | 400 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IHC, FCM |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | O76075 |
| Alias | DFFB, DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase), CAD, CPAN, DFF-40, DFF2, DFF40, DNA fragmentation factor subunit beta |
| Background | Antibody anti-DFFB |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
Background
DNA fragmentation factor subunit beta (DFFB) is an essential component of the apoptotic machinery, functioning as a part of the DNA fragmentation factor (DFF) complex. DFFB forms a heterodimer with its partner subunit, DNA fragmentation factor subunit alpha (DFFA). This beta subunit possesses the enzymatic activity required for the cleavage of DNA into nucleosomal fragments, a hallmark of apoptosis. DFFB, a caspase-activated DNase, is kept in an inactive state by its binding to DFFA until caspases, triggered by apoptotic signals, cleave DFFA, releasing DFFB to execute its DNase function. Dysregulation of DFFB activity is associated with pathological conditions, including resistance to apoptosis in cancer and excessive cell death in degenerative diseases. DFFB activity is tightly regulated to ensure its apoptotic function is confined to specific cellular contexts.
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