Human ATP-Dependent Translocase ABCB1 (ABCB1) Protein

Este producto es parte de ABCB - ATP binding cassette subfamily B member
Human ATP-Dependent Translocase ABCB1 (ABCB1) Protein

Pida mas información

935106861

info@markelab.com

Precio

260€ (10 µg)

Protein ABCB1

proveedor

Abbexa

reference

abx068471

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Human ATP-Dependent Translocase ABCB1 (ABCB1) Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).


Background

ABCB1, also known as P-glycoprotein, is a well-studied ATP-dependent efflux transporter involved in the transport of a wide range of substrates, including drugs, lipids, and xenobiotics. It is highly expressed in barrier tissues, such as the blood-brain barrier, intestines, liver, and kidneys, where it functions to protect tissues by actively exporting toxic compounds and drugs. ABCB1 plays a crucial role in pharmacokinetics, influencing drug absorption, distribution, and elimination. Overexpression of ABCB1 in cancer cells is a major contributor to multidrug resistance, as it pumps chemotherapeutic agents out of cells, reducing their efficacy. Structurally, ABCB1 contains two transmembrane domains and two ATP-binding domains, which utilize ATP hydrolysis to drive substrate transport. Its pivotal role in drug resistance and detoxification makes it a significant target in cancer therapy and pharmacological research.

Características del producto

category

Proteins and Peptides


origin

Human


host

E. coli


immunogen target

ATP-Dependent Translocase ABCB1 (ABCB1)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 57.9 kDa Observed MW (SDS-PAGE): 61 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Phe394-Val672 Tag: N-terminal His tag and GST tag


expression

Recombinant


purity

> 97%


buffer

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

ABCB1


dry ice

No


availability

Shipped within 5-7 working days.


alias

ABC20,CD243,CLCS,GP170,MDR1,P-GP,PGY1,p-170


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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