Human ATP-Dependent Translocase ABCB1 (ABCB1) Protein

Este producto es parte de ABCB - ATP binding cassette subfamily B member
Human ATP-Dependent Translocase ABCB1 (ABCB1) Protein
260€ (10 µg)

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Name
Human ATP-Dependent Translocase ABCB1 (ABCB1) Protein
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx068471
Tested Applications
WB, SDS-PAGE

Description

Human ATP-Dependent Translocase ABCB1 (ABCB1) Protein is a recombinant Human protein expressed in E. coli.

Documentos del producto

Instrucciones
Data sheet
Descargar

Especificaciones del producto

Category
Proteins and Peptides
Immunogen Target
ATP-Dependent Translocase ABCB1 (ABCB1)
Host
E. coli
Assay Type
Activity: Not tested
Sequence Fragment: Phe394-Val672
Tag: N-terminal His tag and GST tag
Origin
Human
Conjugation
Unconjugated
Observed MW
Calculated MW: 57.9 kDa  Observed MW (SDS-PAGE): 61 kDa
Expression
Recombinant
Purity
> 97%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Dry Ice
No
UniProt ID
P08183
Gene ID
5243
OMIM
171050
Alias
ABC20,CD243,CLCS,GP170,MDR1,P-GP,PGY1,p-170
Background
Protein ABCB1
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 200 µg/ml

Background

ABCB1, also known as P-glycoprotein, is a well-studied ATP-dependent efflux transporter involved in the transport of a wide range of substrates, including drugs, lipids, and xenobiotics. It is highly expressed in barrier tissues, such as the blood-brain barrier, intestines, liver, and kidneys, where it functions to protect tissues by actively exporting toxic compounds and drugs. ABCB1 plays a crucial role in pharmacokinetics, influencing drug absorption, distribution, and elimination. Overexpression of ABCB1 in cancer cells is a major contributor to multidrug resistance, as it pumps chemotherapeutic agents out of cells, reducing their efficacy. Structurally, ABCB1 contains two transmembrane domains and two ATP-binding domains, which utilize ATP hydrolysis to drive substrate transport. Its pivotal role in drug resistance and detoxification makes it a significant target in cancer therapy and pharmacological research.

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