Human Insulin-like Growth Factor Binding Protein 3 (IGFBP-3) LIA

Product specifications

ELISA Kits

Human

Incubation Period: 3.5h
Calibrator: native IGFBP-3
Controls: 2 lyophilisedControls

Functional

0.18 – 15150 µg/L

0.18 µg/L

1:505

Antibody: Rabbit

96 det.

Elisa

Serum
EDTA / Heparin Plasma

3-5 days.

Shelf life: between 1 and 2 years
Microtiterplate and reagents are stable until the indicated expiry

growth hormone-dependent binding protein, acid stable subunit of the 140 K IGF complex,IBP3,BP-53,IBP-3,IGFBP-3

Elisa Kits for: IGFBP3

RUO

Intra/Interassay Variance [%]<10

Descripción

Kit de inmunoensayo adecuado para medir la unión bioactiva y funcional de IGF-I (IGFBP-3) humana. Junto con las mediciones correspondientes en las mismas muestras de IGFBP-3 total (tIGFBP-3) con los respectivos kits Mediagnost E03, IGF-R10 o IGF-R11, se puede determinar el grado de fragmentación de IGFBP-3.

All currently existing IGFBP-3 immunoassays use the binding of specific anti-IGFBP-3 antibodies for signal generation and thus IGFBP-3 quantification. The failure of differentiation between complete IGFBP-3 molecules and their respective fragments (derived physiologically due to the different proteases activities) is unavoidable in this system. Because one molecule IGFBP-3 can be cleaved in several fragments often false high quantitative values are measured. Based on this methodology it is not possible to differentiate between high IGFBP-3 levels in fact, or, a high degree of fragmentation. The incidental attempts to use monoclonal antibodies with a binding region represented only by the intact IGFBP-3 molecule are indirect, imprecise and insufficient. The activities of all effective proteases, which have different sites of action and therefore generate different kind of fragments, are disregarded. The Mediagnost IGFBP-3 LIA however enables to determine the real functional and effective bioactive IGFBP-3, functional in terms of binding ability of the mainly interesting natural ligand, namely IGF-I.The new test principle (patent pending DE19719001) uses anti-IGFBP-3 antibodies immobilized on the microtitre plate and biotinylated IGF-I as ligand. The IGFBP-3 of the sample is bound to the microtitre plate, and only concomitant bound, by IGFBP-3, biotinylated IGF-I gives the specific signal of the test. Therefore only functional IGFBP-3 is quantificated.

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