DNA Fragmentation Factor Subunit Beta (DFFB) Antibody
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Description
DFFB Antibody is a Rabbit Polyclonal against DFFB.
Documents del producto
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | DNA Fragmentation Factor Subunit Beta (DFFB) |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Recommended Dilution | ELISA: 1 µg/ml, WB: 1/200 - 1/1000, IHC-P: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified by affinity chromatography. |
| Size 1 | 20 µl |
| Size 2 | 100 µl |
| Size 3 | 2 × 100 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IHC |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | O76075 |
| Gene ID | 1677 |
| NCBI Accession | NP_004393.1 |
| Alias | DFFB, DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase), CAD, CPAN, DFF-40, DFF2, DFF40, DNA fragmentation factor subunit beta |
| Background | Antibody anti-DFFB |
| Status | RUO |
| Note | Concentration: > 0.2 mg/ml - |
Descripción
DNA fragmentation factor subunit beta (DFFB) is an essential component of the apoptotic machinery, functioning as a part of the DNA fragmentation factor (DFF) complex. DFFB forms a heterodimer with its partner subunit, DNA fragmentation factor subunit alpha (DFFA). This beta subunit possesses the enzymatic activity required for the cleavage of DNA into nucleosomal fragments, a hallmark of apoptosis. DFFB, a caspase-activated DNase, is kept in an inactive state by its binding to DFFA until caspases, triggered by apoptotic signals, cleave DFFA, releasing DFFB to execute its DNase function. Dysregulation of DFFB activity is associated with pathological conditions, including resistance to apoptosis in cancer and excessive cell death in degenerative diseases. DFFB activity is tightly regulated to ensure its apoptotic function is confined to specific cellular contexts.
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