anti-Pseudomonas aeruginosa IgG ELISA

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Description
The Mediagnost Anti-Pseudomonas aeruginosa IgG ELISA (Ref: E15), is an enzyme immunoassay for qualitative and quantitative detection of IgG-Antibodies in human serum against the extracellular proteins: Alkaline Protease (AP), Elastase (ELA) and Exotoxin A (Exo A) of Pseudomonas aeruginosa.
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Product specifications
| Category | ELISA Kits |
| Reactivity | Human |
| Assay Data | Reference Material: not available Incubation Period: 4.5 h Calibrator: positive human serum |
| Assay Type | sandwich |
| Test Range | 0.2 – 2500 [titer/mL] |
| Sensitivity | < 0.2 [Titre/mL] |
| Recommended Dilution | 1:1000/ 1:10000/ 1:100000 |
| Origin | Antibody: alkaline protease Elastase Exotoxin A |
| Size 1 | 3 x 96 det. |
| Tested Applications | Elisa |
| Sample Type | Serum / EDTA plasma |
| Availability | 3-5 days. |
| Storage | Shelf life: between 1 and 2 years. Store the kit at 2-8°C until its expiry date. |
| Background | Elisa Kits for: Pseudomonas |
| Status | IVD |
| Note | Intra/Interassay Variance [%]< 20% for titers > 1250 |
Descripción
Anti-Pseudomonas aeruginosa IgG Elisa kit de Mediagnost (Ref: E15), es un inmunoensayo enzimático para la detección cualitativa y cuantitativa de anticuerpos IgG en suero humano contra las proteínas extracelulares: proteasa alcalina (AP), elastasa (ELA) y exotoxina A (Exo A) de Pseudomonas aeruginosa.
Pseudomonas aeruginosa, a Gram-negative bacterium ubiquitously distributed in the moist environment, causes about 10% of all nosocomial infections. This opportunistic pathogen leads to acute and chronic types of infection within various organs of susceptible patient groups. Chronic pulmonary infection in patients with cystic fibrosis (CF) is very frequent, may start early in infancy and determines the life expectancy of these patients. P. aeruginosa infection provokes a production of antibodies to a large number of P. aeruginosa antigens in CF patients. Mediagnost’s sensitive antibody detection system is an aid to discriminate between infected and uninfected patient groups. Thus, the test is a supplement of microbiological diagnostics, which can be influenced by sampling technique (e.g. throat swab). Depending on the Pseudomonas aeruginosa species and the patient’s immune reaction, antibodies can be detected against a single, two or even all three antigens simultaneously. A patient is regarded as sero-positive when the serum is positive for one or more of the antigens.
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