Hepatitis A-Virus Antigen (HAV) ELISA
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Description
Enzyme immunoassay for the qualitative detection of hepatitis A virus antigen in human stool specimens.Early detection of HAV antigen in stool samples from patients with gastrointestinal disease allows rapid establishment of hygiene measures, preventing further spread of the virus.
Documents del producto
Product specifications
| Category | ELISA Kits |
| Reactivity | Human |
| Assay Data | Reference Material: NIBSC 95/500 Incubation Period: 4.5h Calibrator: positive / negative control |
| Assay Type | Sandwich |
| Recommended Dilution | 1:05 |
| Origin | Antibody: Mouse |
| Size 1 | 96 det. |
| Tested Applications | Elisa |
| Sample Type | Faeces / Cell Culture Media |
| Availability | 3-5 days. |
| Storage | Shelf life: between 1 and 2 years. All materials must be stored at 2 - 8°C in the dark |
| Alias | HAVAg |
| Background | Elisa Kits for: HAV |
| Status | RUO |
| Note | Intra/Interassay Variance [%] |
Descripción
Inmunoensayo enzimático para la detección cualitativa del antígeno del virus de la hepatitis A en muestras de heces humanas. La detección temprana del antígeno del VHA en muestras de heces de pacientes con enfermedades gastrointestinales permite establecer rápidamente medidas de higiene, previniendo una mayor propagación del virus.
A positive identification of Hepatitis A Virus (HAV) in human stool samples indicates a fresh and contagious infection with HAV. The passing begins about two weeks before the icteric phase of the disease and reached a peak after about one week before icterus. With the beginning of the icteric phase, the HAV passing drops steeply but HAV antigen could be found in the stool of some, not all, humans two weeks after onset of icterus. The detection of HAV in specimen other than stool is also possible with the mediagnost HAV-ANTIGEN ELISA E12, for example in lysates of HAV infected cells or in culture supernatants. If neccessary the specimen must be concentrated before testing
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Anti-HAV ELISA
Mediagnost anti-HAV ELISA, (Ref: E10) is an enzyme immunoassay for the detection of antibodies against Hepatitis A virus in Human Serum for research use.
Ver ProductoAnti-HAV IgM ELISA
The Mediagnost anti-HAV IgM ELISA is a "class-capture" enzyme immuoassay. Serum or plasma samples are diluted 1:2000 and added to the wells of a microtiter plate, which have been previously coated with an antibody directed against human IgM antibodies (µ-chain specific). All IgM antibodies present in the sample are bound during an incubation step. After washing, the Hepatitis A-virus is added, which binds only to the HAV-specific IgM antibodies. The bound antigen is detected by the addition of the conjugate (monoclonal anti-HAV antibody, peroxidase conjugated). After washing and incubation with a colorimetric substrate the reaction is terminated by addition of stop solution and the color turns to yellow. The absorbance of the coloured reaction product is measured on a microtiter plate reader. The colour intensity of the reaction corresponds to the concentration of antibodies in the sample
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