Anti-HAV ELISA
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Description
Mediagnost anti-HAV ELISA, (Ref: E10) is an enzyme immunoassay for the detection of antibodies against Hepatitis A virus in Human Serum for research use.
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Product specifications
| Category | ELISA Kits |
| Reactivity | Human |
| Assay Data | Reference Material: WHO 97/646 Incubation Period: 3.5h Calibrator: human IgG Controls: 2 controls ready for use |
| Assay Type | pseudo-competitive |
| Test Range | 0–50 IU/L |
| Recommended Dilution | 1:10 |
| Origin | Antibody: Mouse / Human |
| Size 1 | 96 det. |
| Tested Applications | Elisa |
| Sample Type | Serum EDTA / Heparin Plasma |
| Availability | 3-5 days. |
| Storage | Shelf life: between 1 and 2 years. Store the kit at 2-8°C after receipt until its expiry date |
| Alias | HAVIgG |
| Background | Elisa Kits for: HAV IgG |
| Status | RUO |
| Note | Intra/Interassay Variance [%]<30 |
Descripción
Mediagnost anti-HAV ELISA, (Ref: E10) es un inmunoensayo enzimático para la detección de anticuerpos contra el virus de la hepatitis A en suero humano para uso en investigació
After infection with the Hepatitis A-Virus, neutralising antibodies appear at the same time of AntiHAV of IgG-Class formation. The Anti-HAV titers 3 to 6 months after naturally occurring infections are very high, within the range of 100 to more than 300 International Units per ml (IU/ml). Even after more than 10 years the titers usually remain at more than 1 to 10 IU/ml. A value of 10 - 30 (mIU/ml) milli International Units can be considered the minimal protective level. Since the incidence of HAV infection in children has diminished in Northern Europe in recent years, children and juveniles are predominantly Anti-HAV negative. In an adult population could be observed, that the percentage of anti-HAV positive individuals raises with increasing age: only 3.9 % of the young people (18-24 years) show antibody against HAV, where as in older population (≥50 years) HAV-Antibody was found in 40.3 % of the population. In countries around the Mediterranean area, Africa or Asia, the incidence is very high.
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Anti-HAV IgM ELISA
The Mediagnost anti-HAV IgM ELISA is a "class-capture" enzyme immuoassay. Serum or plasma samples are diluted 1:2000 and added to the wells of a microtiter plate, which have been previously coated with an antibody directed against human IgM antibodies (µ-chain specific). All IgM antibodies present in the sample are bound during an incubation step. After washing, the Hepatitis A-virus is added, which binds only to the HAV-specific IgM antibodies. The bound antigen is detected by the addition of the conjugate (monoclonal anti-HAV antibody, peroxidase conjugated). After washing and incubation with a colorimetric substrate the reaction is terminated by addition of stop solution and the color turns to yellow. The absorbance of the coloured reaction product is measured on a microtiter plate reader. The colour intensity of the reaction corresponds to the concentration of antibodies in the sample
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Enzyme immunoassay for the qualitative detection of hepatitis A virus antigen in human stool specimens.Early detection of HAV antigen in stool samples from patients with gastrointestinal disease allows rapid establishment of hygiene measures, preventing further spread of the virus.
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