DFFA antibody
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935106861
info@markelab.com
name
DFFA antibody
category
Primary Antibodies
provider
FineTest
reference
FNab02347
tested applications
ELISA, WB, IHC, IP, IF
Documents del producto
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA) |
| Host | Rabbit |
| Reactivity | Human |
| Recommended Dilution | WB: 1:500-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200 |
| Clonality | polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Observed MW | 45kd |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Size 1 | 100µg |
| Form | liquid |
| Tested Applications | ELISA, WB, IHC, IP, IF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| UniProt ID | O00273 |
| Alias | DFF-45, DFF1, ICAD, Inhibitor of caspase-activated DNase,DFF45,DFFA |
| Background | Antibody anti-DFFA |
| Status | RUO |
| Note | Mol. Weight 45 kDa |
DNA fragmentation factor subunit alpha (DFFA) acts as the regulatory component of the DFF complex, forming a heterodimer with DFFB. DFFA functions primarily as an inhibitor of DFFB, maintaining it in an inactive state in healthy cells. Upon receiving apoptotic signals, caspases cleave DFFA, thereby releasing and activating DFFB. This cleavage event is a critical checkpoint in the apoptotic pathway, ensuring that DNA fragmentation occurs only during apoptosis. Beyond its role as a DFFB inhibitor, DFFA is implicated in nuclear condensation, which occurs concurrently with DNA fragmentation. Alterations in DFFA expression or function can influence the cell's ability to undergo programmed cell death, impacting both normal tissue homeostasis and disease processes, such as tumorigenesis or immune-related disorders.
