SERPINF2 - serpin family F member 2 |Elisa - Clia - Antibody - Protein

Family main features

Background

SERPINF2, also known as alpha-2 antiplasmin (A2AP), is a crucial member of the serpin (serine protease inhibitor) family. It plays a vital role in regulating the fibrinolytic system by inhibiting plasmin, the enzyme responsible for breaking down fibrin clots. As part of the serpin superfamily, SERPINF2 helps to maintain hemostasis by preventing excessive clot breakdown, ensuring that clots remain stable during wound healing and tissue repair.

First characterized in the 1970s, SERPINF2 has since been extensively studied for its role in clot stabilization and its involvement in various bleeding disorders and thrombotic conditions. It is primarily synthesized in the liver and circulates in the plasma, where it acts as a major inhibitor of fibrinolysis.


Protein Structure

The SERPINF2 protein is composed of 464 amino acids, with a molecular weight of approximately 70 kDa. Like other serpins, its structure features a central β-sheet and multiple α-helices. The reactive center loop (RCL), a hallmark of serpins, plays a crucial role in its inhibitory mechanism. The RCL of SERPINF2 interacts with plasmin to form a covalent complex, thereby inactivating the enzyme and preventing the degradation of fibrin clots.

Upon binding to plasmin, SERPINF2 undergoes a conformational change that traps plasmin in a covalent complex, rendering it inactive. This interaction is critical for ensuring that fibrinolysis is tightly regulated and that clots are broken down only when necessary.

SERPINF2 also contains a lysine-binding site, which facilitates its interaction with fibrin, allowing it to localize to sites of clot formation and provide targeted inhibition of plasmin activity. This ensures that clot breakdown occurs in a controlled manner, preventing premature clot dissolution and excessive bleeding.


Classification and Subtypes

SERPINF2 belongs to the clade F of the serpin superfamily, which is characterized by its role in inhibiting enzymes involved in fibrinolysis. While it shares structural similarities with other serpins, such as alpha-1 antitrypsin and SERPINF1, its function is distinct due to its specificity for plasmin.

There are no known subtypes of SERPINF2, but genetic variants and mutations can affect its function and lead to clinical conditions. These variants may alter the stability or inhibitory activity of SERPINF2, resulting in either an increased risk of bleeding (due to insufficient plasmin inhibition) or a predisposition to thrombosis (due to excessive clot stabilization).


Function and Biological Significance

The primary function of SERPINF2 is to regulate the fibrinolytic system by inhibiting plasmin, the enzyme responsible for breaking down fibrin clots. By doing so, it plays a crucial role in maintaining the balance between clot formation and clot breakdown, ensuring that clots remain stable during the initial stages of wound healing and are only dissolved once tissue repair is complete.

  1. Inhibition of Plasmin: Plasmin is the main enzyme responsible for degrading fibrin, the protein that forms the structural framework of blood clots. By inhibiting plasmin, SERPINF2 prevents premature clot dissolution, allowing clots to remain intact during the healing process. This is particularly important in situations where stable clot formation is essential, such as after surgery or injury.
  2. Clot Stabilization: In addition to inhibiting plasmin, SERPINF2 also binds to fibrin, the main component of blood clots, which allows it to localize to sites of clot formation. This targeted inhibition ensures that plasmin activity is restricted to areas where clot breakdown is necessary, preventing excessive bleeding while maintaining the integrity of the clot.
  3. Role in Hemostasis: Hemostasis is the process by which the body stops bleeding and repairs damaged blood vessels. SERPINF2 plays a key role in this process by regulating fibrinolysis, ensuring that clots remain stable during the early stages of healing and are only broken down when tissue repair is complete. This helps to prevent excessive blood loss and ensures that the healing process proceeds smoothly.
  4. Wound Healing: Beyond its role in clot stabilization, SERPINF2 also contributes to wound healing by preventing excessive plasmin activity, which can degrade not only fibrin but also other extracellular matrix proteins. By inhibiting plasmin, SERPINF2 helps to preserve the structural integrity of the extracellular matrix, allowing for proper tissue repair.


Clinical Issues

Mutations or deficiencies in the SERPINF2 gene can lead to several clinical conditions, primarily related to abnormal clotting or bleeding. The most notable conditions associated with SERPINF2 dysfunction include:

  1. Alpha-2 Antiplasmin Deficiency: This rare inherited disorder is characterized by a deficiency of functional SERPINF2, leading to excessive plasmin activity and premature clot breakdown. Individuals with this condition are at an increased risk of bleeding, particularly after surgery or injury. Symptoms may include easy bruising, prolonged bleeding, and recurrent nosebleeds. Severe cases can result in life-threatening bleeding episodes.
  2. There are two types of alpha-2 antiplasmin deficiency: congenital (inherited) and acquired. Congenital deficiency is caused by mutations in the SERPINF2 gene, while acquired deficiency can result from conditions such as liver disease or disseminated intravascular coagulation (DIC), where SERPINF2 is consumed or degraded at an accelerated rate.
  3. Thrombotic Disorders: While SERPINF2 deficiency is primarily associated with bleeding disorders, dysregulation of its activity can also contribute to thrombotic conditions. Excessive inhibition of plasmin activity by SERPINF2 can lead to reduced clot breakdown, increasing the risk of thrombosis (the formation of abnormal blood clots). This can result in conditions such as deep vein thrombosis (DVT), pulmonary embolism, or stroke.
  4. Liver Disease: Since SERPINF2 is primarily synthesized in the liver, liver dysfunction can lead to reduced production of this protein, resulting in an increased risk of bleeding. In conditions such as cirrhosis or liver failure, decreased SERPINF2 levels may contribute to coagulopathy, a condition where the blood’s ability to clot is impaired.


Therapeutic Applications

Given its role in regulating fibrinolysis, SERPINF2 has potential therapeutic applications in conditions where abnormal clotting or bleeding occurs. For example, recombinant SERPINF2 may be used to treat individuals with alpha-2 antiplasmin deficiency, helping to restore normal clot stabilization and prevent excessive bleeding.

In addition, inhibitors of SERPINF2 activity could be explored as potential therapies for thrombotic conditions, where excessive clot stabilization contributes to the development of abnormal blood clots. By reducing SERPINF2 activity, it may be possible to promote controlled clot breakdown and reduce the risk of thrombosis.


Summary

SERPINF2, or alpha-2 antiplasmin, is a critical regulator of the fibrinolytic system, ensuring that blood clots remain stable during wound healing and are only broken down when tissue repair is complete. By inhibiting plasmin, SERPINF2 prevents premature clot dissolution and plays a key role in maintaining hemostasis.

Mutations or deficiencies in SERPINF2 can lead to bleeding disorders, such as alpha-2 antiplasmin deficiency, where excessive plasmin activity results in uncontrolled clot breakdown and increased bleeding. On the other hand, dysregulation of SERPINF2 activity can contribute to thrombotic conditions by reducing the breakdown of abnormal blood clots.

Given its essential role in clot regulation, SERPINF2 has potential therapeutic applications in both bleeding and thrombotic disorders, making it a key target for further research and clinical development.


SERPINF2 Recommended name:

serpin family F member 2 (SERPINF2)

Aliases for SERPINF2

AAP,API,PLI,A2AP,alpha2AP,Alpha-2-antiplasmin,Alpha-2-AP,Alpha-2-plasmin inhibitor,Serpin F2

En la tabla siguiente se muestra una comparativa de todos los reactivos disponibles en nuestro catálogo (Proteins and Peptides, Primary Antibodies, ELISA Kits, CLIA Kits) relacionados con SERPINF2 - serpin family F member 2

Se muestran ordenados por categorías para poder comparar cómodamente sus características principales. Esta tabla, que contiene un enlace con la ficha de cada producto, es exportable a Excel.

Esta página contiene 27 reactivos de las marcas (Abbexa, FineTest) que se corresponden con tu busqueda

Contacta con nosotros en info@markelab.com, si necesitas mas informacion o alguna aclaracion. Te garantizamos respuesta en menos de 24 h.

immunoassays

providerCodereferencenamereactivitysample typeassay typetest rangesensitivitypricesize 1uniprot idstatus
AbbexaSERPINF2abx575395Cow Alpha-2 Antiplasmin (SERPINF2) ELISA KitCowSerum,Plasma,Other biological fluids0.312 ng/ml - 20 ng/ml687.596 testsP28800RUO
AbbexaSERPINF2abx491162Human Alpha-2 Antiplasmin (SERPINF2) CLIA KitHumanSerum, plasma and other biological fluids.Sandwich1.56 ng/ml - 100 ng/ml< 0.71 ng/ml812.596 testsRUO
AbbexaSERPINF2abx253372Human Alpha-2 Antiplasmin (SERPINF2) ELISA KitHumanSerum,Plasma,Other biological fluidsSandwich12.5 ng/ml - 800 ng/ml7.5 ng/ml52596 testsP08697RUO
AbbexaSERPINF2abx150491Human Alpha-2 Antiplasmin (SERPINF2) ELISA KitHumanSerum,Plasma,Other biological fluidsSandwich0.156 ng/ml - 10 ng/ml< 0.07 ng/ml618.7596 testsRUO
AbbexaSERPINF2abx197904Human Alpha-2 Antiplasmin (SERPINF2) CLIA KitHumanSerum, plasma and other biological fluids.0.31 ng/ml - 20 ng/ml0.19 ng/ml618.7596 testsRUO
AbbexaSERPINF2abx585311Mouse Alpha-2 Antiplasmin (SERPINF2) ELISA KitMouseSerum,Plasma,Other biological fluidsSandwich0.312 ng/ml - 20 ng/ml< 0.127 ng/ml618.7596 testsRUO
AbbexaSERPINF2abx196709Mouse Alpha-2 Antiplasmin (SERPINF2) CLIA KitMouseSerum, plasma and other biological fluids.1.56 ng/ml - 100 ng/ml0.94 ng/ml618.7596 testsRUO
AbbexaSERPINF2abx254509Mouse Alpha-2 Antiplasmin (SERPINF2) ELISA KitMouseSerum,Plasma,Other biological fluidsSandwich3.125 ng/ml - 200 ng/ml1.88 ng/ml562.596 testsRUO
AbbexaSERPINF2abx362359Rabbit Alpha-2 Antiplasmin (SERPINF2) ELISA KitRabbitSerum, plasma and other biological fluids.Sandwich0.47 ng/ml - 30 ng/ml0.29 ng/ml687.596 testsRUO
AbbexaSERPINF2abx576086Rat Alpha-2 Antiplasmin (SERPINF2) ELISA KitRatSerum,Plasma,Other biological fluidsSandwich3.125 ng/ml - 200 ng/ml1.88 ng/ml52596 testsQ80ZA3RUO
AbbexaSERPINF2abx353984Rat Alpha-2 Antiplasmin (SERPINF2) ELISA KitRatPlasma.Sandwich3.12 ng/ml - 200 ng/ml< 1.28 ng/ml67596 testsRUO

Primary Antibodies

providerCodereferencenamereactivityclonalityhostimmunogen targetisotypeconjugationtested applicationspricesize 1uniprot idstatus
AbbexaSERPINF2abx022448alpha 2 Antiplasmin (SERPINF2) AntibodyMonoclonalMousealpha 2 Antiplasmin (SERPINF2)UnconjugatedELISA, WB2137.51 mgRUO
AbbexaSERPINF2abx102505Alpha-2 Antiplasmin (SERPINF2) AntibodyHumanPolyclonalRabbitAlpha-2 Antiplasmin (SERPINF2)UnconjugatedWB, IHC, IF/ICC250100 µlP08697RUO
AbbexaSERPINF2abx432316alpha 2-Antiplasmin (SERPINF2) AntibodyHumanPolyclonalGoatalpha 2-Antiplasmin (SERPINF2)UnconjugatedP-ELISA, WB, IHC, IF/ICC, FCM387.5200 µlRUO
AbbexaSERPINF2abx211298Alpha-2 Antiplasmin (SERPINF2) AntibodyHumanPolyclonalRabbitAlpha-2 Antiplasmin (SERPINF2)UnconjugatedELISA, WB, IHC25050 µlP08697RUO
AbbexaSERPINF2abx230339Alpha-2 Antiplasmin (SERPINF2) AntibodyHumanPolyclonalRabbitAlpha-2 Antiplasmin (SERPINF2)UnconjugatedELISA, WB350100 µgP08697RUO
AbbexaSERPINF2abx115475Alpha-2 Antiplasmin (SERPINF2) AntibodyHumanPolyclonalRabbitAlpha-2 Antiplasmin (SERPINF2)UnconjugatedELISA, WB612.5100 µlP08697RUO
AbbexaSERPINF2abx102506Alpha-2 Antiplasmin (SERPINF2) AntibodyMousePolyclonalRabbitAlpha-2 Antiplasmin (SERPINF2)UnconjugatedWB, IHC, IF/ICC262.5100 µlRUO
AbbexaSERPINF2abx135865Alpha-2 Antiplasmin (SERPINF2) AntibodyMousePolyclonalRabbitAlpha-2 Antiplasmin (SERPINF2)UnconjugatedWB, IHC17520 µlP08697RUO
AbbexaSERPINF2abx102507Alpha-2 Antiplasmin (SERPINF2) AntibodyRatPolyclonalRabbitAlpha-2 Antiplasmin (SERPINF2)UnconjugatedWB, IHC, IF/ICC262.5100 µlRUO

Proteins and Peptides

providerCodereferencenameoriginexpressionhostconjugationtested applicationspricesize 1uniprot idstatus
AbbexaSERPINF2abx065261Mouse Alpha-2 Antiplasmin (SERPINF2) ProteinMouseRecombinantE. coliUnconjugatedWB, SDS-PAGE22510 µgQ61247RUO
FineTestSERPINF2P0511Recombinant Human SERPINF2HumanRecombinantE.ColiWestern Blot, ELISA50μgP08697RUO
AbbexaSERPINF2abx694273Mouse Alpha-2-Antiplasmin (SERPINF2) ProteinMouseRecombinantHEK293 cellsSDS-PAGE30020 µgQ61247RUO
AbbexaSERPINF2abx065260Human Alpha-2 Antiplasmin (SERPINF2) ProteinHumanRecombinantE. coliUnconjugatedWB, SDS-PAGE22510 µgP08697RUO
AbbexaSERPINF2abx691905Mouse alpha 2-Antiplasmin (SerpinF2) ProteinMouseRecombinantHEK293 cellsSDS-PAGE97550 µgQ61247RUO
AbbexaSERPINF2abx680468Mouse Alpha-2 Antiplasmin (SERPINF2) ProteinMouseRecombinantInsectUnconjugatedSDS-PAGE2252 µgRUO
AbbexaSERPINF2abx065262Rat Alpha-2 Antiplasmin (SERPINF2) ProteinRatRecombinantE. coliUnconjugatedWB, SDS-PAGE237.510 µgQ68FT8RUO

Te recomendamos que si no encuentras lo que buscas, utilices el buscador, refinando la búsqueda según tu criterio y usando Alias, o bien contacta con nosotros.