Rat Proteinase-Activated Receptor 2 / PAR2 (F2RL1) Protein

Este producto es parte de F2R - Proteinase-Activated Receptor
Rat Proteinase-Activated Receptor 2 / PAR2 (F2RL1) Protein
208€ (10 µg)

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Name
Rat Proteinase-Activated Receptor 2 / PAR2 (F2RL1) Protein
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx654829
Tested Applications
WB, SDS-PAGE

Description

Rat Proteinase-Activated Receptor 2 / PAR2 (F2RL1) Protein is a recombinant Rat protein expressed in E. coli.

Documentos del producto

Instrucciones
Data sheet
Descargar

Especificaciones del producto

Category
Proteins and Peptides
Immunogen Target
Proteinase-Activated Receptor 2 / PAR2 (F2RL1)
Host
E. coli
Assay Type
Activity: Not tested
Sequence Fragment: Val309-Tyr397
Tag: N-terminal His and GST tags
Origin
Rat
Conjugation
Unconjugated
Observed MW
Calculated MW: 40 kDa  Observed MW: 42 kDa
Expression
Recombinant
Purity
> 90%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Dry Ice
No
Alias
F2RL1,GPR11,coagulation factor II receptor-like 1,Protease-activated receptor-2 ,thrombin receptor-like 1,PAR2
Background
Protein F2RL1
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 200 µg/ml

Background

Proteinase-Activated Receptor 2 (F2RL1), also known as PAR2, is a G protein-coupled receptor (GPCR) activated by the cleavage of its N-terminal domain by serine proteases, such as trypsin and mast cell tryptase. PAR2 is widely expressed in various tissues, including endothelial cells, platelets, smooth muscle, and immune cells, where it regulates inflammatory responses, cell migration, and pain perception. Upon activation, F2RL1 couples to Gq, Gi, and G12/13 proteins, stimulating phospholipase C (PLC), MAPK, and Rho signaling pathways. These pathways lead to increased intracellular calcium, cell contraction, and gene expression associated with inflammation. F2RL1 plays a role in immune cell recruitment during allergic reactions, wound healing, and tissue remodeling. Dysregulation of F2RL1 has been implicated in inflammatory diseases, cancer metastasis, and pain disorders. PAR2 antagonists are being developed for therapeutic applications in conditions like asthma, chronic pain, and cancer, by modulating its pro-inflammatory effects and enhancing tissue repair.

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