Rat Polyunsaturated Fatty Acid 5-Lipoxygenase (ALOX5) Protein
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935106861
info@markelab.com
Precio
221€ (10 µg)
Protein ALOX5
proveedor
Abbexareference
abx652159Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Arachidonate-5-Lipoxygenase (ALOX5) Protein is a Recombinant protein from Rat.
Background
ALOX5 is a key enzyme in the biosynthesis of leukotrienes, potent lipid mediators involved in inflammation, immune responses, and allergic reactions. ALOX5 catalyzes the oxidation of arachidonic acid to form 5-hydroperoxyeicosatetraenoic acid (5-HPETE), which is subsequently converted into leukotriene A4 (LTA4), the precursor for leukotrienes B4 (LTB4), C4 (LTC4), D4, and E4. These leukotrienes play significant roles in recruiting neutrophils, promoting vascular permeability, and inducing smooth muscle contraction. ALOX5 is highly expressed in immune cells such as neutrophils, eosinophils, and macrophages, where it mediates inflammatory responses to infection, injury, and allergens. Dysregulation of ALOX5 is associated with asthma, rheumatoid arthritis, atherosclerosis, and cancer, where leukotrienes amplify inflammation and promote tissue damage. In cancer, ALOX5 enhances tumor cell proliferation, survival, and angiogenesis through lipid signaling. As such, ALOX5 inhibitors, including zileuton, are used therapeutically for asthma and are being explored for anti-cancer treatments targeting lipid metabolism.
Características del producto
category
Proteins and Peptides
origin
Rat
host
E. coli
immunogen target
Polyunsaturated Fatty Acid 5-Lipoxygenase (ALOX5)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 20 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Leu373-Leu535 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
ALOX5
dry ice
No
availability
Shipped within 5-12 working days.
alias
ALOX5,LOG5
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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