Mouse Polyunsaturated Fatty Acid 5-Lipoxygenase (ALOX5) Protein

Este producto es parte de ALOX - Arachidonate lipoxygenase
Mouse Polyunsaturated Fatty Acid 5-Lipoxygenase (ALOX5) Protein

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935106861

info@markelab.com

Precio

221€ (10 µg)

Protein ALOX5

proveedor

Abbexa

reference

abx065451

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Recombinant Arachidonate-5-Lipoxygenase (ALOX5) is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).


Background

ALOX5 is a key enzyme in the biosynthesis of leukotrienes, potent lipid mediators involved in inflammation, immune responses, and allergic reactions. ALOX5 catalyzes the oxidation of arachidonic acid to form 5-hydroperoxyeicosatetraenoic acid (5-HPETE), which is subsequently converted into leukotriene A4 (LTA4), the precursor for leukotrienes B4 (LTB4), C4 (LTC4), D4, and E4. These leukotrienes play significant roles in recruiting neutrophils, promoting vascular permeability, and inducing smooth muscle contraction. ALOX5 is highly expressed in immune cells such as neutrophils, eosinophils, and macrophages, where it mediates inflammatory responses to infection, injury, and allergens. Dysregulation of ALOX5 is associated with asthma, rheumatoid arthritis, atherosclerosis, and cancer, where leukotrienes amplify inflammation and promote tissue damage. In cancer, ALOX5 enhances tumor cell proliferation, survival, and angiogenesis through lipid signaling. As such, ALOX5 inhibitors, including zileuton, are used therapeutically for asthma and are being explored for anti-cancer treatments targeting lipid metabolism.

Características del producto

category

Proteins and Peptides


origin

Mouse


host

E. coli


immunogen target

Polyunsaturated Fatty Acid 5-Lipoxygenase (ALOX5)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 23.0 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Val223-Lys410 Tag: N-terminal His tag


expression

Recombinant


purity

> 95%


buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

ALOX5


dry ice

No


availability

Shipped within 5-12 working days.


alias

ALOX5,LOG5


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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reactivity

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