Rat Alanine Glyoxylate Aminotransferase (AGXT) Protein

234€ (10 µg)
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935106861
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name
Rat Alanine Glyoxylate Aminotransferase (AGXT) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx168880
tested applications
WB, SDS-PAGE
Description
Rat AGXT Protein is a recombinant Rat protein produced in a Prokaryotic expression system (E. coli).
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | Alanine Glyoxylate Aminotransferase (AGXT) |
| Host | E. coli |
| Origin | Rat |
| Conjugation | Unconjugated |
| Observed MW | Molecular Weight: Calculated MW: 25.1 kDa Observed MW (SDS-PAGE): 25 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Thr182-Gly371 Tag: N-terminal His tag |
| Expression | Recombinant |
| Purity | > 95% |
| Size 1 | 10 µg |
| Size 2 | 50 µg |
| Size 3 | 100 µg |
| Size 4 | 200 µg |
| Size 5 | 500 µg |
| Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300. |
| Availability | Shipped within 5-7 working days. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P09139 |
| Alias | AGXT, AGT1, SPAT |
| Background | Protein AGXT |
| Status | RUO |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
Alanine-glyoxylate aminotransferase (AGXT) is a pyridoxal phosphate-dependent enzyme localized in peroxisomes, where it plays a critical role in detoxifying glyoxylate by converting it into glycine, using alanine as an amino donor. AGXT is central to preventing the overproduction of oxalate, a byproduct of glyoxylate metabolism, which, if accumulated, can form calcium oxalate crystals leading to kidney stones and renal damage. Mutations in the AGXT gene cause primary hyperoxaluria type 1 (PH1), a rare autosomal recessive disorder characterized by excessive oxalate production, kidney stone formation, and systemic oxalosis. AGXT's activity is regulated by intracellular localization and metabolic cues, and therapies targeting AGXT include enzyme replacement therapy and gene therapy to restore glyoxylate metabolism in affected individuals.
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