Rat Acrosin (ACR) Protein
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935106861
info@markelab.com
Precio
234€ (10 µg)
Protein ACR
proveedor
Abbexareference
abx065106Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Recombinant Acrosin (ACR) is a recombinant Rat protein produced in a Prokaryotic expression system (E. coli).
Background
Acrosin (ACR) is a serine protease enzyme localized in the acrosome of spermatozoa, where it plays a critical role in the fertilization process. It is synthesized as an inactive zymogen called proacrosin, which is activated during the acrosome reaction to assist in the penetration of the sperm through the zona pellucida, the outer glycoprotein layer of the oocyte. Acrosin functions by hydrolyzing zona pellucida proteins and facilitating sperm binding to the egg, making it essential for successful fertilization. The activity of acrosin is tightly regulated to prevent premature activation, ensuring that it is only active during the fertilization process. Dysregulation or mutations affecting acrosin function have been associated with male infertility due to impaired sperm-oocyte interaction. Acrosin is also studied as a marker for sperm acrosomal integrity and functionality in reproductive biology and clinical diagnostics. Research into acrosin inhibitors is being explored as a potential strategy for developing non-hormonal contraceptives.
Características del producto
category
Proteins and Peptides
origin
Rat
host
E. coli
immunogen target
Acrosin (ACR)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 29.1 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Ile43-Gly291 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
ACR
dry ice
No
availability
Shipped within 5-12 working days.
alias
SPGF87
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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