Mouse Sirtuin 3 (SIRT3) Protein

Este producto es parte de SIRT3 - Sirtuin 3
Mouse Sirtuin 3 (SIRT3) Protein

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935106861

info@markelab.com

Precio

650€ (50 µg)

Protein SIRT3

proveedor

Abbexa

reference

abx651440

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Sirtuin 3 (SIRT3) Protein (Active) is an active protein from Mouse.


Background

SIRT3 is a NAD+-dependent deacetylase primarily localized in the mitochondria, where it plays a critical role in regulating mitochondrial metabolism, energy production, and oxidative stress response SIRT3 deacetylates and activates key mitochondrial enzymes, including acetyl-CoA synthetase 2 (ACSS2), superoxide dismutase 2 (SOD2), and components of the electron transport chain, enhancing ATP production, fatty acid oxidation, and antioxidant defense SIRT3 is essential for maintaining mitochondrial homeostasis during energy stress, such as fasting or caloric restriction, by stimulating pathways that improve energy efficiency and reduce reactive oxygen species (ROS) levels SIRT3 also promotes cellular survival under oxidative stress by deacetylating SOD2, which neutralizes superoxide radicals Dysregulation of SIRT3 is associated with aging, metabolic disorders, cardiovascular disease, and neurodegeneration, where impaired mitochondrial function leads to cellular damage and energy deficiency SIRT3 has tumor suppressor properties as it inhibits aerobic glycolysis (Warburg effect) in cancer cells by regulating key metabolic enzymes Its activation is being explored as a therapeutic strategy to combat mitochondrial dysfunction, improve metabolic health, and delay aging-related diseases

Características del producto

category

Proteins and Peptides


origin

Mouse


host

E. coli


immunogen target

Sirtuin 3 (SIRT3)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

50 µg


size 2

100 µg


size 3

200 µg


size 4

500 µg


size 5

1 mg


observerd MW

Molecular Weight: Calculated MW: 32.2 kDa Observed MW: 16 kDa, 29 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Gly5-Arg257 Tag: N-terminal His tag


purity

> 90%


buffer

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

SIRT3


dry ice

No


availability

Shipped within 5-7 working days.


alias

SIR2L3,NAD-dependent protein deacetylase sirtuin-3 mitochondrial,SIR2-like protein 3,SIR2L3,hSIRT3


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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