Mouse Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein

Este producto es parte de GAPDH - Glyceraldehyde-3-phosphate dehydrogenase
Mouse Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein

Pida mas información

935106861

info@markelab.com

Precio

234€ (10 µg)

Protein GAPDH

proveedor

Abbexa

reference

abx066863

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Recombinant GAPDH (GAPDH) is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).


Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme predominantly involved in glycolysis, catalyzing the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate using NAD+ as a cofactor. Beyond its metabolic role, GAPDH participates in various non-metabolic processes, including transcriptional regulation, DNA repair, and cell apoptosis. Its involvement in oxidative stress response and as a target for S-nitrosylation connects it to neurodegenerative diseases such as Alzheimer's and Parkinson's. Furthermore, GAPDH can translocate to the nucleus under stress conditions, influencing apoptotic pathways.

Características del producto

category

Proteins and Peptides


origin

Mouse


host

E. coli


immunogen target

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 25.7 kDa Observed MW (SDS-PAGE): 26 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Gly56-Phe284 Tag: N-terminal His tag


expression

Recombinant


purity

> 95%


buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

GAPDH


dry ice

No


availability

Shipped within 5-7 working days.


alias

GAPDH, GAPD, G3PD, HEL-S-162eP,Peptidyl-cysteine S-nitrosylase GAPDH


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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