Mouse Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein
234€ (10 µg)
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935106861
info@markelab.com
name
Mouse Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx066863
tested applications
WB, SDS-PAGE
Description
Recombinant GAPDH (GAPDH) is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) |
| Host | E. coli |
| Origin | Mouse |
| Conjugation | Unconjugated |
| Observed MW | Molecular Weight: Calculated MW: 25.7 kDa Observed MW (SDS-PAGE): 26 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Gly56-Phe284 Tag: N-terminal His tag |
| Expression | Recombinant |
| Purity | > 95% |
| Size 1 | 10 µg |
| Size 2 | 50 µg |
| Size 3 | 100 µg |
| Size 4 | 200 µg |
| Size 5 | 500 µg |
| Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300. |
| Availability | Shipped within 5-7 working days. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P16858 |
| Alias | GAPDH, GAPD, G3PD, HEL-S-162eP,Peptidyl-cysteine S-nitrosylase GAPDH |
| Background | Protein GAPDH |
| Status | RUO |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme predominantly involved in glycolysis, catalyzing the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate using NAD+ as a cofactor. Beyond its metabolic role, GAPDH participates in various non-metabolic processes, including transcriptional regulation, DNA repair, and cell apoptosis. Its involvement in oxidative stress response and as a target for S-nitrosylation connects it to neurodegenerative diseases such as Alzheimer's and Parkinson's. Furthermore, GAPDH can translocate to the nucleus under stress conditions, influencing apoptotic pathways.
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