Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody
Pida mas información
935106861
info@markelab.com
Precio
383.5€ (100 µl)
Antibody anti-GAPDH
proveedor
Abbexareference
abx010853Tested Applications
ELISA, WB, IHC, IF/ICCreactivity
Humanstatus
RUOclonality
MonoclonalDescripción
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme predominantly involved in glycolysis, catalyzing the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate using NAD+ as a cofactor. Beyond its metabolic role, GAPDH participates in various non-metabolic processes, including transcriptional regulation, DNA repair, and cell apoptosis. Its involvement in oxidative stress response and as a target for S-nitrosylation connects it to neurodegenerative diseases such as Alzheimer's and Parkinson's. Furthermore, GAPDH can translocate to the nucleus under stress conditions, influencing apoptotic pathways.
Características del producto
category
Primary Antibodies
clonality
Monoclonal
reactivity
Human
immunogen target
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
host
Mouse
isotype
IgG1
conjugation
Unconjugated
form
Liquid
tested applications
ELISA, WB, IHC, IF/ICC
purification
Unpurified ascites.
recommended dilution
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, IF/ICC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
buffer
Ascitic fluid containing 0.03% sodium azide.
size 1
100 µl
storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
or code
GAPDH
dry ice
No
availability
Shipped within 5-10 working days.
alias
GAPDH, GAPD, G3PD, HEL-S-162eP,Peptidyl-cysteine S-nitrosylase GAPDH
uniprot id
note
Concentration: Not determined. -
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