Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody
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935106861
info@markelab.com
Precio
260€ (100 µl)
Antibody anti-GAPDH
proveedor
Abbexareference
abx005568Tested Applications
WB, IHC, IF/ICC, IPreactivity
Human, Mouse, Ratstatus
RUOclonality
PolyclonalDescripción
GAPDH Antibody is a Rabbit Polyclonal antibody against GAPDH. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transport, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PACR and also loading control in electrophoresis and Western blotting. This antibody is a rabbit polyclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme predominantly involved in glycolysis, catalyzing the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate using NAD+ as a cofactor. Beyond its metabolic role, GAPDH participates in various non-metabolic processes, including transcriptional regulation, DNA repair, and cell apoptosis. Its involvement in oxidative stress response and as a target for S-nitrosylation connects it to neurodegenerative diseases such as Alzheimer's and Parkinson's. Furthermore, GAPDH can translocate to the nucleus under stress conditions, influencing apoptotic pathways.
Características del producto
category
Primary Antibodies
clonality
Polyclonal
reactivity
Human, Mouse, Rat
immunogen target
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
host
Rabbit
isotype
IgG
conjugation
Unconjugated
form
Liquid
tested applications
WB, IHC, IF/ICC, IP
purification
Purified by affinity chromatography.
recommended dilution
WB: 1/10000 - 1/30000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
buffer
PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
size 1
100 µl
size 2
2 × 100 µl
storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
or code
GAPDH
dry ice
No
availability
Shipped within 5-10 working days.
alias
GAPDH, GAPD, G3PD, HEL-S-162eP,Peptidyl-cysteine S-nitrosylase GAPDH
uniprot id
NCBI accession
NP_002037.2
note
Concentration: > 0.2 mg/ml -
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