Human NAD-Dependent Protein Deacetylase Sirtuin-3, Mitochondrial (SIRT3) Protein
559€ (50 µg)
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935106861
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name
Human NAD-Dependent Protein Deacetylase Sirtuin-3, Mitochondrial (SIRT3) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx655827
tested applications
WB, SDS-PAGE
Description
Human NAD-Dependent Protein Deacetylase Sirtuin-3, Mitochondrial (SIRT3) Protein (Active) is an Active recombinant Human protein.
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | NAD-Dependent Protein Deacetylase Sirtuin-3, Mitochondrial (SIRT3) |
| Host | E. coli |
| Assay Type | Activity: Active Biological Activity: Sirtuin 3 (SIRT3), an NAD-dependent deacetylase, is a member of the mammalian sirtuin family of proteins. In humans, sirtuins have a range of molecular functions and have emerged as important proteins in ageing, stress resistance and metabolic regulation. It also can regulate epigenetic gene silencing and suppress recombination of rDNA in yeast. SIRT3 is expressed in white and brown adipose tissue. Isocitrate Dehydrogenase 2, mitochondrial (IDH2) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SIRT3 and recombinant human IDH2. Briefly, SIRT3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to IDH2-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-SIRT3 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. Sequence Fragment: Gln126-Lys399 Tag: N-terminal His tag |
| Origin | Human |
| Conjugation | Unconjugated |
| Observed MW | Calculated MW: 31.7 kDa Observed MW (SDS-PAGE): 32 kDa |
| Expression | Recombinant |
| Purity | > 92% |
| Size 1 | 50 µg |
| Size 2 | 100 µg |
| Size 3 | 200 µg |
| Size 4 | 500 µg |
| Size 5 | 1 mg |
| Form | Lyophilized |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: 100 mM NaHCO<sub>3</sub>, 500 mM NaCl, pH 8.3, containing 0.01% sarcosyl, 5% trehalose. |
| Availability | Shipped within 5-7 working days. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Dry Ice | No |
| UniProt ID | Q9NTG7 |
| Gene ID | 23410 |
| Alias | SIR2L3,NAD-dependent protein deacetylase sirtuin-3 mitochondrial,SIR2-like protein 3,SIR2L3,hSIRT3 |
| Background | Protein SIRT3 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. Reconstitute in ddH2O to a concentration between 0.1-1.0 mg/ml. Do not vortex. Concentration: Prior to lyophilization: 200 µg/ml |
Descripción
SIRT3 is a NAD+-dependent deacetylase primarily localized in the mitochondria, where it plays a critical role in regulating mitochondrial metabolism, energy production, and oxidative stress response SIRT3 deacetylates and activates key mitochondrial enzymes, including acetyl-CoA synthetase 2 (ACSS2), superoxide dismutase 2 (SOD2), and components of the electron transport chain, enhancing ATP production, fatty acid oxidation, and antioxidant defense SIRT3 is essential for maintaining mitochondrial homeostasis during energy stress, such as fasting or caloric restriction, by stimulating pathways that improve energy efficiency and reduce reactive oxygen species (ROS) levels SIRT3 also promotes cellular survival under oxidative stress by deacetylating SOD2, which neutralizes superoxide radicals Dysregulation of SIRT3 is associated with aging, metabolic disorders, cardiovascular disease, and neurodegeneration, where impaired mitochondrial function leads to cellular damage and energy deficiency SIRT3 has tumor suppressor properties as it inhibits aerobic glycolysis (Warburg effect) in cancer cells by regulating key metabolic enzymes Its activation is being explored as a therapeutic strategy to combat mitochondrial dysfunction, improve metabolic health, and delay aging-related diseases
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