Human Rhodopsin (RHO) Protein

Este producto es parte de OPN - Opsin receptors
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208€ (10 µg)

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935106861
info@markelab.com
name
Human Rhodopsin (RHO) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx654957
tested applications
WB, SDS-PAGE

Description

Human Rhodopsin (RHO) Protein is a Recombinant Human protein expressed in E. coli.

Documents del producto

Instrucciones
Data sheet
Descargar

Product specifications

Category
Proteins and Peptides
Immunogen Target
Rhodopsin (RHO)
Host
E. coli
Origin
Human
Conjugation
Unconjugated
Observed MW
Molecular Weight: Calculated MW: 22.2 kDa  Observed MW (SDS-PAGE): 27 kDa  Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

Concentration: Prior to lyophilization: 200 µg/ml

Sequence Fragment: Met1-Asn73

Tag: N-terminal His tag and SUMO tag
Expression
Recombinant
Purity
> 95%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Availability
Shipped within 5-7 working days.
Storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
P08100
Gene ID
6010
Alias
RHO,RP4,OPN2,CSNBAD1
Background
Protein RHO
Status
RUO
Note
This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

Rhodopsin (RHO) is a light-sensitive G protein-coupled receptor found in the rod cells of the retina, which plays a crucial role in vision, especially in low-light conditions. It is composed of the protein opsin and a retinal chromophore. When light hits rhodopsin, the retinal undergoes a conformational change, activating the opsin and initiating a signaling cascade that results in hyperpolarization of the rod cell and the transmission of visual signals to the brain. Rhodopsin is highly sensitive to light, making it essential for scotopic vision, or vision in dim light. Mutations in the RHO gene can lead to various retinal diseases, including retinitis pigmentosa, which causes progressive vision loss and, eventually, blindness. Research into rhodopsin and its mechanisms is important for understanding retinal diseases and developing potential therapies to treat them, including gene therapies and pharmacological interventions to restore or protect vision.

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