Human Polyunsaturated Fatty Acid Lipoxygenase ALOX15 (ALOX15) Protein
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935106861
info@markelab.com
Precio
247€ (10 µg)
Protein ALOX15
proveedor
Abbexareference
abx065449Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Human Arachidonate-15-Lipoxygenase (ALOX15) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Background
ALOX15 catalyzes the oxygenation of arachidonic acid and linoleic acid to generate 15(S)-hydroperoxyeicosatetraenoic acid [15(S)-HPETE] and 13(S)-hydroxyoctadecadienoic acid (13(S)-HODE), respectively. These lipid intermediates are precursors for resolvins, protectins, and other specialized pro-resolving mediators (SPMs) involved in the resolution of inflammation. ALOX15 is highly expressed in reticulocytes, macrophages, and epithelial cells, where it plays roles in redox regulation, membrane remodeling, and anti-inflammatory responses. In macrophages, ALOX15 promotes the clearance of apoptotic cells (efferocytosis) and contributes to tissue homeostasis during inflammation resolution. Dysregulation of ALOX15 is associated with various pathological conditions, including asthma, atherosclerosis, diabetes, and neurodegenerative diseases. In cancer biology, ALOX15 has dual roles, acting as both a tumor suppressor by inhibiting proliferation and a pro-tumorigenic factor in specific contexts. Its ability to generate lipid mediators essential for resolving inflammation highlights its therapeutic potential in chronic inflammatory and metabolic disorders.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Polyunsaturated Fatty Acid Lipoxygenase ALOX15 (ALOX15)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 17.3 kDa Observed MW: 17 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Lys170-Leu312 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
ALOX15
dry ice
No
availability
Shipped within 5-7 working days.
alias
ALOX15,LOG15
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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