Human Non Catalytic Region Of Tyrosine Kinase Adaptor Protein 1 (NCK1) Protein

Este producto es parte de NCK adaptor protein
Human Non Catalytic Region Of Tyrosine Kinase Adaptor Protein 1 (NCK1) Protein
234€ (10 µg)

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Name
Human Non Catalytic Region Of Tyrosine Kinase Adaptor Protein 1 (NCK1) Protein
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx167977
Tested Applications
WB, SDS-PAGE

Description

Human Non Catalytic Region Of Tyrosine Kinase Adaptor Protein 1 is a recombinant Human protein expressed in E. coli.

Documentos del producto

Instrucciones
Data sheet
Descargar

Especificaciones del producto

Category
Proteins and Peptides
Immunogen Target
Non Catalytic Region Of Tyrosine Kinase Adaptor Protein 1 (NCK1)
Host
E. coli
Assay Type
Activity: Not tested
Sequence Fragment: Ala2-Ser377
Tag: N-terminal His tag
Origin
Human
Conjugation
Unconjugated
Observed MW
Calculated MW: 46.4 kDa
Expression
Recombinant
Purity
> 90%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Dry Ice
No
Alias
NCK1, NCK, NCKalpha, nck-1, NCK adaptor protein 1
Background
Protein NCK1
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 200 µg/ml

Background

NCK1 is a cytoplasmic adaptor protein containing three SH3 domains and one SH2 domain, which allows it to mediate interactions between receptor tyrosine kinases (RTKs) and downstream effectors in signaling pathways. NCK1 is involved in actin cytoskeleton reorganization, cell motility, and immune synapse formation by linking signaling proteins such as WASP, WAVE, and PAK1. It plays key roles in processes like cell migration, proliferation, and T-cell activation. NCK1 is highly expressed in various tissues and regulates growth factor receptor signaling, including pathways like MAPK/ERK and PI3K. Dysregulation of NCK1 is associated with cancer progression, metastasis, and immune system dysfunction. Knockout studies show defects in actin dynamics, impaired cell movement, and reduced T-cell activation, highlighting its importance in cytoskeletal signaling and cellular communication.

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