Human Insulin-like Growth Factor-II (IGF-II) ELISA

Product specifications

ELISA Kits

Human

Reference Material: NIBSC 96/538
Incubation Period: 3 h
Calibrator: recombinant IGF-II
Controls: 2 Controls, lyophilised

Sandwich

0.06 – 3636 µg/L

0.06 µg/L

1:404

Antibody: Mouse / Goats

96 det.

Elisa

Serum
EDTA / Heparin Plasma

3-5 days.

Shelf life: between 1 and 2 years
Store the kit at 2-8°C after receipt until its expiry date

Mpr,M6pr,Peg2,Igf-2,Igf-II

Elisa Kits for: IGF2

IVD

Intra/Interassay Variance [%]<10

Descripción

Kit de inmunoensayo enzimático adecuado para medir IGF-II, Human Insulin-like Growth Factor-II, en suero y plasma humanos con fines diagnósticos y científicos.

The insulin-like growth factors (IGF)-I and –II play a pivotal role in the regulation of proliferation and differentiation of several tissue types. IGF-I also called Somatomedin C  has a molecular weight of 7.469 kDa. Its expression is mainly regulated by Growth Hormone and nutrition. But several hormones and peptide factors are known to influence IGF-II synthesis in different tissues. Bioavailability of the IGFs is regulated by specific binding proteins (IGFBP). Beside the high affinity Insulin-like Growth Factor Binding Proteins 1-6, IGFs are also bound be IGFBP-related Proteins. These binding proteins bind IGF-I and IGF-II with the same affinity or prefer IGF-II. Direct measurement of IGFs in serum samples without pretreatment results in false values because of the extremely slow dissociation of the IGF/IGFBP complexes during the assay incubation only a part of the IGFII in the specimen can bind to the antibodies and be detected.
This assay is easy, fast and results do not depend on the binding protein concentration of the sample. It is based on the high specificity of the employed antibodies for IGF-II. There is virtually no cross-reactivity with IGF-I. This allows the separation of IGF-II from the binding proteins by acidification and blocking of the free binding proteins with IGF-I. Thus, the endogenous IGF-II is free in solution

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