Human Diphthamide Biosynthesis Protein 2 (DPH2) ELISA Kit
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Description
Human Diphthamide Biosynthesis Protein 2 (DPH2) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human DPH2 concentrations in serum, plasma and other biological fluids.
Documents del producto
Product specifications
| Category | ELISA Kits |
| Immunogen Target | Diphthamide Biosynthesis Protein 2 (DPH2) |
| Reactivity | Human |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| Assay Type | Sandwich |
| Test Range | 0.156 ng/ml - 10 ng/ml |
| Sensitivity | < 0.094 ng/ml |
| Recommended Dilution | Optimal dilutions/concentrations should be determined by the end user. |
| Size 1 | 96 tests |
| Form | Lyophilized |
| Tested Applications | ELISA |
| Sample Type | Serum, plasma and other biological fluids. |
| Availability | Shipped within 5-12 working days. The validity for this kit is 6 months. |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Dry Ice | No |
| UniProt ID | Q9BQC3 |
| Gene ID | 1802 |
| OMIM | 603456 |
| Alias | DPH2L2,DEDSSH2,Diphthamide biosynthesis protein 2,Diphtheria toxin resistance protein 2 |
| Background | Elisa kits for DPH2 |
| Status | RUO |
| Note | Validity: The validity for this kit is 6 months. This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
Descripción
DPH2 is an essential enzyme that works in concert with DPH1 to catalyze the first step of diphthamide biosynthesis on eukaryotic elongation factor 2 (eEF2), forming a critical intermediate for subsequent modifications. It interacts directly with DPH1 to mediate the transfer of a 3-amino-3-carboxypropyl group from S-adenosylmethionine (SAM) to the imidazole ring of a specific histidine residue. DPH2 mutations are associated with defects in protein translation fidelity and resistance to diphtheria toxin, as this toxin specifically targets diphthamide. Structural studies of DPH2 reveal conserved binding sites for SAM, emphasizing its central role in catalytic function. In addition to its enzymatic role, DPH2 is required for maintaining normal cellular physiology, particularly in protein synthesis and stress responses. DPH2 expression is tightly regulated and primarily localized in the cytoplasm, where it coordinates with other diphthamide biosynthesis factors. Deficiencies in DPH2 activity result in incomplete diphthamide synthesis, impacting cellular growth and proliferation. Its evolutionary conservation highlights its critical role in cellular and organismal viability.
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