DPH2 antibody
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Description
Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2(EEF2).
Documents del producto
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | DPH2 homolog (DPH2) |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Recommended Dilution | WB: 1:500-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200 |
| Clonality | polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Observed MW | 52kd |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Size 1 | 100µg |
| Form | liquid |
| Tested Applications | ELISA, WB, IHC, IP, IF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| UniProt ID | Q9BQC3 |
| Gene ID | 1802 |
| Alias | DPH2L2,DEDSSH2,Diphthamide biosynthesis protein 2,Diphtheria toxin resistance protein 2 |
| Background | Antibody anti-DPH2 |
| Status | RUO |
| Note | Mol. Weight 52 kDa |
Descripción
DPH2 is an essential enzyme that works in concert with DPH1 to catalyze the first step of diphthamide biosynthesis on eukaryotic elongation factor 2 (eEF2), forming a critical intermediate for subsequent modifications. It interacts directly with DPH1 to mediate the transfer of a 3-amino-3-carboxypropyl group from S-adenosylmethionine (SAM) to the imidazole ring of a specific histidine residue. DPH2 mutations are associated with defects in protein translation fidelity and resistance to diphtheria toxin, as this toxin specifically targets diphthamide. Structural studies of DPH2 reveal conserved binding sites for SAM, emphasizing its central role in catalytic function. In addition to its enzymatic role, DPH2 is required for maintaining normal cellular physiology, particularly in protein synthesis and stress responses. DPH2 expression is tightly regulated and primarily localized in the cytoplasm, where it coordinates with other diphthamide biosynthesis factors. Deficiencies in DPH2 activity result in incomplete diphthamide synthesis, impacting cellular growth and proliferation. Its evolutionary conservation highlights its critical role in cellular and organismal viability.
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Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2(EEF2).
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