Human Dedicator Of Cytokinesis 4 (DOCK4) Protein
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935106861
info@markelab.com
Precio
221€ (10 µg)
Protein DOCK4
proveedor
Abbexareference
abx168105Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Human Dedicator Of Cytokinesis 4 Protein is a recombinant Human protein expressed in E. coli.
Background
DOCK4 is a GEF that activates Rac1 and Rap1, regulating cell adhesion, migration, and junction formation. It is critical for maintaining epithelial integrity and angiogenesis by promoting actin cytoskeletal remodeling. DOCK4 is widely expressed in tissues, including the brain, and has roles in neuronal development, synapse formation, and dendritic growth. Mutations in DOCK4 are linked to cancers, particularly prostate, ovarian, and colorectal cancers, as they disrupt cell adhesion and enhance invasion. It interacts with proteins like ELMO1 for Rac1 activation and mediates processes such as cell spreading. Knockdown studies show impaired junction stability and migration, emphasizing its role in tissue organization and metastasis.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Dedicator Of Cytokinesis 4 (DOCK4)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 38.7 kDa Observed MW (SDS-PAGE): 39 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Met1-Pro300 Tag: N-terminal His tag
expression
Recombinant
purity
> 90%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
DOCK4
dry ice
No
availability
Shipped within 5-7 working days.
alias
DOCK4, KIAA0716
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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