Human Dedicator Of Cytokinesis 4 (DOCK4) Protein

221€ (10 µg)
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935106861
info@markelab.com
name
Human Dedicator Of Cytokinesis 4 (DOCK4) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx168105
tested applications
WB, SDS-PAGE
Description
Human Dedicator Of Cytokinesis 4 Protein is a recombinant Human protein expressed in E. coli.
Documents del producto
Instrucciones
Data sheet
Product specifications
Category | Proteins and Peptides |
Immunogen Target | Dedicator Of Cytokinesis 4 (DOCK4) |
Host | E. coli |
Origin | Human |
Conjugation | Unconjugated |
Observed MW | Molecular Weight: Calculated MW: 38.7 kDa Observed MW (SDS-PAGE): 39 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Met1-Pro300 Tag: N-terminal His tag |
Expression | Recombinant |
Purity | > 90% |
Size 1 | 10 µg |
Size 2 | 50 µg |
Size 3 | 100 µg |
Size 4 | 200 µg |
Size 5 | 500 µg |
Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Tested Applications | WB, SDS-PAGE |
Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300. |
Availability | Shipped within 5-7 working days. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
Dry Ice | No |
UniProt ID | Q8N1I0 |
Gene ID | 9732 |
OMIM | 607679 |
Alias | DOCK4, KIAA0716 |
Background | Protein DOCK4 |
Status | RUO |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
DOCK4 is a GEF that activates Rac1 and Rap1, regulating cell adhesion, migration, and junction formation. It is critical for maintaining epithelial integrity and angiogenesis by promoting actin cytoskeletal remodeling. DOCK4 is widely expressed in tissues, including the brain, and has roles in neuronal development, synapse formation, and dendritic growth. Mutations in DOCK4 are linked to cancers, particularly prostate, ovarian, and colorectal cancers, as they disrupt cell adhesion and enhance invasion. It interacts with proteins like ELMO1 for Rac1 activation and mediates processes such as cell spreading. Knockdown studies show impaired junction stability and migration, emphasizing its role in tissue organization and metastasis.
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