Human Caldesmon (CALD1) Protein

Este producto es parte de CALD1 - Caldesmon
Human Caldesmon (CALD1) Protein

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935106861

info@markelab.com

Precio

221€ (10 µg)

Protein CALD1

proveedor

Abbexa

reference

abx065663

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Human Caldesmon (CALD) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).

Background

CALD1 (Caldesmon 1) is a versatile actin-binding protein predominantly expressed in smooth muscle and non-muscle cells, where it plays a key role in modulating cytoskeletal dynamics and cellular contractility. CALD1 interacts with actin filaments, myosin, tropomyosin, and calmodulin, acting as a regulatory mediator of actomyosin interactions crucial for cellular processes such as contraction, migration, and shape changes. In smooth muscle cells, CALD1 contributes to the regulation of contraction by inhibiting the ATPase activity of myosin in a calcium-calmodulin-dependent manner, thereby modulating muscle tone and elasticity. In non-muscle cells, it is implicated in stabilizing the cytoskeleton and facilitating cell motility, often in response to signaling cascades that control cell migration and adhesion. CALD1 has also been associated with cancer progression, where its expression is frequently altered, contributing to tumor invasiveness and metastasis by influencing cytoskeletal reorganization and cellular dynamics. Its phosphorylation state, regulated by various kinases, fine-tunes its interaction with actin and myosin, enabling precise control over cytoskeletal remodeling. These multifunctional attributes make CALD1 a critical player in maintaining cellular integrity and adaptability across different physiological and pathological contexts.

Características del producto

category

Proteins and Peptides

origin

Human

host

E. coli

immunogen target

Caldesmon (CALD1)

host

E. coli

conjugation

Unconjugated

form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.

tested applications

WB, SDS-PAGE

size 1

10 µg

size 2

50 µg

size 3

100 µg

size 4

200 µg

size 5

500 µg

observerd MW

Molecular Weight: Calculated MW: 25.1 kDa Observed MW (SDS-PAGE): 38 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. Concentration: Prior to lyophilization: 120 µg/ml Sequence Fragment: Gln28-Gln207 Tag: N-terminal His tag

expression

Recombinant

purity

> 97%

buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.

storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

or code

CALD1

dry ice

No

availability

Shipped within 5-7 working days.

alias

CALD1, CDM, H-CAD, HCAD, L-CAD caldesmon

uniprot id

https://www.uniprot.org/uniprotkb/Q05682

gene id

https://www.ncbi.nlm.nih.gov/gene/800

NCBI accession

NP_004333.1, NM_004342.6, NP_149129.2, NM_033138.3

note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

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