Human ATP Binding Cassette Transporter A1 (ABCA1) Protein
234€ (10 µg)
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Name
Human ATP Binding Cassette Transporter A1 (ABCA1) Protein
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx168177
Tested Applications
WB, SDS-PAGE
Description
Human ATP Binding Cassette Transporter A1 Protein is a recombinant Human protein expressed in E. coli.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Proteins and Peptides |
| Immunogen Target | ATP Binding Cassette Transporter A1 (ABCA1) |
| Host | E. coli |
| Assay Type | Activity: Not tested Sequence Fragment: Asn1385-Phe1663 Tag: N-terminal His tag |
| Origin | Human |
| Conjugation | Unconjugated |
| Observed MW | Calculated MW: 34.5 kDa Observed MW (SDS-PAGE): 36 kDa |
| Expression | Recombinant |
| Purity | > 97% |
| Size 1 | 10 µg |
| Size 2 | 50 µg |
| Size 3 | 100 µg |
| Size 4 | 200 µg |
| Size 5 | 500 µg |
| Form | Lyophilized |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Availability | Shipped within 5-7 working days. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Dry Ice | No |
| Alias | TGD,ABC1,CERP,ABC-1,HDLDT1,HPALP1,HDLCQTL13 |
| Background | Protein ABCA1 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. Concentration: Prior to lyophilization: 200 µg/ml |
Background
ABCA1 is a transmembrane protein belonging to the ATP-binding cassette (ABC) transporter family, playing a vital role in lipid metabolism and cellular cholesterol efflux. It facilitates the transport of cholesterol and phospholipids from cells to apolipoproteins, such as ApoA-I, in the plasma, which is a critical step in the formation of high-density lipoprotein (HDL). Structurally, ABCA1 consists of two transmembrane domains and two ATP-binding domains that utilize ATP hydrolysis to drive lipid transport. It is highly expressed in macrophages, hepatocytes, and other cells involved in lipid metabolism. The activity of ABCA1 is essential for maintaining cellular cholesterol homeostasis and preventing lipid accumulation, particularly in macrophages, where its dysfunction can lead to foam cell formation and atherosclerosis. Mutations in the ABCA1 gene are associated with Tangier disease, a rare disorder characterized by very low HDL levels and increased susceptibility to cardiovascular disease. As a key regulator of cholesterol efflux and HDL biogenesis, ABCA1 is a therapeutic target for managing dyslipidemia and cardiovascular risk.
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