Human A Disintegrin And Metalloprotease 15 (ADAM15) Protein

234€ (10 µg)
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name
Human A Disintegrin And Metalloprotease 15 (ADAM15) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx065058
tested applications
WB, SDS-PAGE
Description
Human A Disintegrin And Metalloprotease 15 (ADAM15) Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Documents del producto
Instrucciones
Data sheet
Product specifications
Category | Proteins and Peptides |
Immunogen Target | A Disintegrin And Metalloprotease 15 (ADAM15) |
Host | E. coli |
Origin | Human |
Conjugation | Unconjugated |
Observed MW | Molecular Weight: Calculated MW: 27.0 kDa Observed MW (SDS-PAGE): 21 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Ser505-Arg745 Tag: N-terminal His tag |
Expression | Recombinant |
Purity | > 95% |
Size 1 | 10 µg |
Size 2 | 50 µg |
Size 3 | 100 µg |
Size 4 | 200 µg |
Size 5 | 500 µg |
Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Tested Applications | WB, SDS-PAGE |
Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose. |
Availability | Shipped within 5-7 working days. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
Dry Ice | No |
UniProt ID | Q13444 |
Alias | MDC15,MDC-15,Metargidin,ADAM-15 |
Background | Protein ADAM15 |
Status | RUO |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
ADAM15, also known as ADAM metallopeptidase domain 15, is a member of the ADAM (a disintegrin and metalloproteinase) family . These proteins are involved in various biological processes, including cell adhesion, migration, proteolysis, and signaling. ADAM15 is a transmembrane protein, meaning it spans the cell membrane, with both extracellular and intracellular domains.ADAM15 consists of several distinct domains, including a metalloproteinase domain responsible for its proteolytic activity, a disintegrin domain involved in cell-cell and cell-matrix interactions, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and a cytoplasmic tail.ADAM15 is implicated in various physiological and pathological processes, including cell adhesion, migration, proliferation, and signaling. It can cleave cell surface proteins, releasing soluble forms of these proteins into the extracellular environment. Is also involved in cell-cell and cell-matrix interactions. It interacts with various proteins on the cell surface and in the extracellular matrix, including integrins, growth factors, cytokines, and cell adhesion molecules.ADAM15 has been linked to cancer progression and metastasis. Its expression is often elevated in various cancers, including breast, lung, colon, prostate, and pancreatic cancers. ADAM15 can promote tumor cell invasion and metastasis by facilitating cell migration and invasion through the extracellular matrix.
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