E2F Transcription Factor 1 Phospho-Thr433 (E2F1 pT433) Antibody
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Description
E2F1 (pT433) Antibody is a Rabbit Polyclonal against E2F1 (pT433).
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Product specifications
| Category | Primary Antibodies |
| Immunogen Target | E2F Transcription Factor 1 Phospho-Thr433 (E2F1 pT433) |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Assay Data | Modification: Phosphorylation // Target Modification: Thr433 |
| Recommended Dilution | ELISA: 1/5000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified by affinity chromatography. |
| Size 1 | 50 µg |
| Size 2 | 100 µg |
| Form | Liquid |
| Tested Applications | ELISA, WB |
| Buffer | PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | Q01094 |
| Gene ID | 1869 |
| Alias | E2F-1,RBAP1,RBBP3,RBP3,Retinoblastoma-binding protein 3,Retinoblastoma-associated protein 1 |
| Background | Antibody anti-E2F1 |
| Status | RUO |
Descripción
E2F1 is a member of the E2F family of transcription factors, which are critical regulators of cell cycle progression, DNA replication, and apoptosis. E2F1 drives the transition from G1 to S phase by activating genes required for DNA synthesis and cell proliferation, including cyclins and replication factors. It is tightly regulated by the retinoblastoma protein (pRB), which suppresses its activity under growth-inhibitory conditions. E2F1 also induces apoptosis under cellular stress or DNA damage by activating pro-apoptotic genes such as p53 and caspases. Dysregulation of E2F1 contributes to tumorigenesis by promoting uncontrolled proliferation and resistance to cell death. In cancer, overexpression of E2F1 is often observed, correlating with poor prognosis and enhanced tumor growth. Knockout models reveal defects in cell cycle progression and reduced apoptosis, emphasizing its dual role in proliferation and programmed cell death.
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