Zebrafish Interleukin-1 Beta (IL1B) Protein

Proteins and Peptides

Interleukin-1 Beta (IL1B)

E. coli

Activity: Active
Biological Activity: A549 cells were seeded into a 24 well plate at a density of 1 × 105 cells per ml, and left overnight before treatment with different concentrations of recombinant Zebrafish IL1B (this product) for 48 h. IL-8 levels in the cell supernatant were determined by Human Interleukin 8 / IL8 (CXCL8) ELISA Kit (abx050129). IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulation with IL1B. The EC50 was determined to be 16.86 µg/ml.
Sequence Fragment: Met1-Ile272
Tag: N-terminal His Tag

Zebrafish

Unconjugated

Calculated MW: 34.1 kDa  Observed MW (SDS-PAGE): 37 kDa

Recombinant

> 90%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

Q4KMF1

Protein IL1B

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 µg (determined by the LAL method).
Concentration: Prior to lyophilization: 200 µg/ml