Vimentin (VIM) Antibody
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Description
Vimentin, also know as VIM.It is the major subunit protein of the intermediate filaments of mesenchymal cells.It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane.Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets.Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event.This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative.Melanomas, lymphomas and vascular tumors may all stain for Vimentin.Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors.They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
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Product specifications
| Category | Primary Antibodies |
| Immunogen Target | Vimentin (VIM) |
| Host | Mouse |
| Reactivity | Human, Monkey |
| Recommended Dilution | ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Monoclonal |
| Conjugation | Unconjugated |
| Isotype | IgG1 |
| Purification | Unpurified ascites. |
| Size 1 | 100 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IHC |
| Buffer | Ascitic fluid containing 0.03% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| Gene ID | 7431 |
| Background | Antibody anti-Vimentin |
| Status | RUO |
| Note | Concentration: Not determined. - |
Descripción
Related Products
Vimentin (VIM) Antibody
Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets.
Ver Producto
Vimentin (VIM) Antibody
Vimentin, also know as VIM.It is the major subunit protein of the intermediate filaments of mesenchymal cells.It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane.Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets.Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event.This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative.Melanomas, lymphomas and vascular tumors may all stain for Vimentin.Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors.They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
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Vimentin (VIM) Antibody
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
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