Ubiquitin-40S Ribosomal Protein S27a (RPS27A) Antibody

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312€ (60 µl)

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935106861
info@markelab.com
name
Ubiquitin-40S Ribosomal Protein S27a (RPS27A) Antibody
category
Primary Antibodies
provider
Abbexa
reference
abx001644
tested applications
WB, IHC, IF/ICC

Description

Ubiquitin-40S Ribosomal Protein S27a (RPS27A) Antibody is a Rabbit Polyclonal antibody against Ubiquitin-40S Ribosomal Protein S27a (RPS27A). Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008].

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Product specifications

Category
Primary Antibodies
Immunogen Target
Target: Ubiquitin-40S Ribosomal Protein S27a (RPS27A)
Immunogen: Recombinant fusion protein corresponding to human RPS27A
Host
Rabbit
Reactivity
Human, Mouse, Rat
Assay Type
Concentration: 1 mg/ml
Recommended Dilution
WB: 1/500 - 1/2000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Clonality
Polyclonal
Conjugation
Unconjugated
Isotype
IgG
Observed MW
Calculated MW: 17 kDa  Observed MW: 14 kDa
Purification
Purified by affinity chromatography.
Size 1
60 µl
Size 2
120 µl
Size 3
200 µl
Form
Liquid
Tested Applications
WB, IHC, IF/ICC
Buffer
PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Availability
Shipped within 5-10 working days.
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
P62979
Gene ID
6233
NCBI Accession
NP_001170884.1
Background
Antibody anti-RPS27A
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Descripción

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