TopTaq DNA Polymerase Enzyme
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Description
TopTaq DNA Polymerase contains three binding proteins: one binding protein binds to double-stranded DNA template, preventing polymerase activity at room temperature, and the other two binding proteins bind primers, preventing primer-dimer formation. Together, these binding proteins reduce non-specific amplification. The blocking proteins are released from primers and templates during the initial denaturation. This double-blocking method has higher efficiency and specificity than antibody-based or chemically-modified hot start PCR. Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into T-vectors. This kit does not use Taq antibodies, reducing the risk of mammalian DNA contamination, and this kit does not use chemical modification, so a long denaturing step is not required. Genomic DNA fragments can be amplified up to 15 kb. This kit does not contain 2.5 mM dNTPs.
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Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | TopTaq DNA Polymerase |
| Assay Type | Biological Activity: One unit of TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74 °C. |
| Conjugation | Unconjugated |
| Purity | > 99% (SDS-PAGE) |
| Size 1 | 250 U |
| Size 2 | 500 U |
| Size 3 | 3 kU |
| Tested Applications | PCR |
| Buffer | TopTaq DNA Polymerase: 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. <br> 10X TopTaq Buffer: 500 mM Tris-HCl (pH 9.0), 200 mM (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, 20 mM MgSO<sub>4</sub>, other proprietary ingredients. |
| Availability | Shipped within 10-20 working days. |
| Storage | Store at -20 °C for up to 2 years. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| Background | Protein TopTaq DNA Polymerase |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. Endotoxin Level: Functional absence of double and single stranded endonuclease activity. Concentration: 2.5 U/µl |
Descripción
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TopTaq DNA Polymerase Enzyme
TopTaq DNA Polymerase contains three binding proteins: one binding protein binds to double-stranded DNA template, preventing polymerase activity at room temperature, and the other two binding proteins bind primers, preventing primer-dimer formation. Together, these binding proteins reduce non-specific amplification. The blocking proteins are released from primers and templates during the initial denaturation. This double-blocking method has higher efficiency and specificity than antibody-based or chemically-modified hot start PCR. Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into T-vectors. This kit does not use Taq antibodies, reducing the risk of mammalian DNA contamination, and this kit does not use chemical modification, so a long denaturing step is not required. Genomic DNA fragments can be amplified up to 15 kb. This kit does not contain 2.5 mM dNTPs.
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