T4 DNA Ligase (T4 DNA) Enzyme

Proteins and Peptides

T4 DNA Ligase (T4 DNA)

E. coli

Biological Activity: 1U is the amount of T4 DNA Ligase required to ligate greater than 50% of the DNA fragments in a 20 μl ligation reaction system. 6 μg of lambda DNA-Hind III decomposition product reacts at 16°C for 30 minutes.  T4 DNA Ligase can be inhibited by NaCl/KCl at a concentrations greater than 200 mM or by heating at 65°C for 10 min / 70°C for 5 min.

Optimal dilutions/concentrations should be determined by the end user.

55.3 kDa

Recombinant

20 kIU

100 kIU

500 kIU

Liquid

Storage Buffer: 50% glycerol, 20 mM Tris-HCl, pH 7.5, containing 50 mM KCl, 1 mM DTT and 0.1 mM EDTA.<br> Reaction Buffer: 50 mM Tris-HCl, pH 7.5, containing 10 mM MgCl<sub>2</sub>, 10 mM DTT and 1 mM ATP (25°C or incubate at 16°C).

Shipped within 5-10 working days.

Store at 4°C if the entire vial will be used within 4 weeks. Store at -20 °C for long term storage. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.

No

Protein T4 DNA

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Concentration: 400 U/µl