Replication Factor C Subunit 4 (RFC4) Antibody
195€ (20 µl)
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Name
Replication Factor C Subunit 4 (RFC4) Antibody
Category
Primary Antibodies
Provider
Abbexa
Reference
abx004202
Tested Applications
ELISA, WB, IF/ICC, IP
Description
RFC4 Antibody is a Rabbit Polyclonal antibody against RFC4. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Primary Antibodies |
| Immunogen Target | Target: Replication Factor C Subunit 4 (RFC4) Immunogen: Recombinant protein corresponding to RFC4. The exact sequence is proprietary. |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Assay Type | Concentration: > 0.2 mg/ml |
| Recommended Dilution | ELISA: 1 µg/ml, WB: 1/500 - 1/2000, IF/ICC: 1/50 - 1/100, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Observed MW | Calculated MW: 40 kDa Observed MW: 40 kDa |
| Purification | Purified by affinity chromatography. |
| Size 1 | 20 µl |
| Size 2 | 100 µl |
| Size 3 | 2 × 100 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IF/ICC, IP |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P35249 |
| Gene ID | 5984 |
| NCBI Accession | NP_002907.1 |
| Background | Antibody anti-RFC4 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
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