Recombinant Human PRC1

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Documents del producto
Product specifications
Category | Proteins and Peptides |
Host | E.Coli |
Reactivity | Human |
Assay Data | Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex. |
Observed MW | 32.9 kDa |
Expression | 188-446 |
Purity | Greater than 90% as determined by SDS-PAGE. |
Size 1 | 50μg |
Size 2 | 200μg |
Size 3 | 1mg |
Form | Lyophilized powder |
Tested Applications | Western Blot, ELISA |
Buffer | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4. |
Availability | 7 days |
Storage | The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle. |
UniProt ID | O43663 |
Alias | ASE1, PRC1, protein regulator of cytokinesis 1 |
Background | Protein PRC1 |
Status | RUO |
Note | Tag : N-terminal His Tag |
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PRC1 antibody
Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression(PubMed:17409436).
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