Recombinant Human PPT1
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Product specifications
| Category | Proteins and Peptides |
| Host | Mammalian Cells |
| Reactivity | Human |
| Observed MW | 34-41 kDa |
| Expression | Asp28-Gly306 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Size 1 | 10μg |
| Size 2 | 50μg |
| Size 3 | 500μg |
| Size 4 | 1mg |
| Form | Liquid |
| Tested Applications | Western Blot, ELISA |
| Buffer | 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 10% Glycerol, pH 7.5. |
| Availability | 7 days |
| Storage | The protein solution is stable at -80°C for 6 months after receipt, and for 3 months under sterile conditions after opening. Avoid repeated freeze/thaw cycle. |
| UniProt ID | P50897 |
| Alias | Palmitoyl-protein thioesterase 1, PPT-1, Palmitoyl-protein hydrolase 1, PPT1 |
| Background | Protein PPT1 |
| Status | RUO |
| Note | Endotoxin: < 1 EU/µg as determined by LAL test. Tag : C-terminal His Tag |
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Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8 and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.
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