Recombinant Human IPO4

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Product specifications
Category | Proteins and Peptides |
Host | E.Coli |
Reactivity | Human |
Assay Data | Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex. |
Recommended Dilution | ¥ |
Isotype | ¥ |
Clone ID | ¥ |
Observed MW | 42.9 kDa |
Expression | 624-825 |
Purity | Greater than 90% as determined by SDS-PAGE. |
Size 1 | 50μg |
Size 2 | 200μg |
Size 3 | 1mg |
Form | Lyophilized powder |
Tested Applications | Western Blot, ELISA |
Buffer | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4. |
Availability | 7 days |
Storage | The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle. |
UniProt ID | Q8TEX9 |
Alias | Imp4, Imp4b, Importin 4, Importin 4b, Importin-4, Importin-4b, Ipo4, IPO4_HUMAN, Ran binding protein 4, Ran-binding protein 4, RanBP4 |
Background | Protein IPO4 |
Status | RUO |
Note | Tag : N-terminal His-IF2DI Tag |
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Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals(NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex(NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus(By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
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Recombinant Human IPO4
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