Recombinant Human GM-CSF

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935106861
info@markelab.com
name
Recombinant Human GM-CSF
category
Proteins and Peptides
provider
FineTest
reference
P8665
tested applications
Western Blot, ELISA

Documents del producto

Instrucciones
Data sheet
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Product specifications

Category
Proteins and Peptides
Host
HEK293 cells
Reactivity
Human
Assay Data
Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Observed MW
24-27 kDa
Expression
18-144
Purity
Greater than 90% as determined by SDS-PAGE.
Size 1
50μg
Size 2
200μg
Size 3
1mg
Form
Lyophilized powder
Tested Applications
Western Blot, ELISA
Buffer
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4.
Availability
1-2 weeks
Storage
The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
UniProt ID
P04141
Alias
Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim
Background
Protein GM-CSF
Status
RUO
Note
Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is typically 100-500 pg/mL.

Endotoxin: < 1 EU/µg as determined by LAL test.

Tag : C-terminal His Tag

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