Recombinant Human GM-CSF

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935106861
info@markelab.com
name
Recombinant Human GM-CSF
category
Proteins and Peptides
provider
FineTest
reference
P8665
tested applications
Western Blot, ELISA
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Proteins and Peptides |
| Host | HEK293 cells |
| Reactivity | Human |
| Assay Data | Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex. |
| Observed MW | 24-27 kDa |
| Expression | 18-144 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Size 1 | 50μg |
| Size 2 | 200μg |
| Size 3 | 1mg |
| Form | Lyophilized powder |
| Tested Applications | Western Blot, ELISA |
| Buffer | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4. |
| Availability | 1-2 weeks |
| Storage | The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle. |
| UniProt ID | P04141 |
| Alias | Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim |
| Background | Protein GM-CSF |
| Status | RUO |
| Note | Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is typically 100-500 pg/mL. Endotoxin: < 1 EU/µg as determined by LAL test. Tag : C-terminal His Tag |
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